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DOI :
10.3791/2468-v
•
8:53 min
February 17th, 2011
Chapters
0:05
Title
2:01
Preparation of Cell Line with an Integrated Recombination Substrate
3:04
Transfection to Deplete Endogenous BRCA1 and add back Mutant BRCA1
5:19
Analysis of Transfectants
6:07
Analysis of the Effects of BRCA1 Mutations on Homologous Recombination via GFP-Expression
7:54
Conclusion
우리는 RNAi를 사용하여 세포의 내생 BRCA1 단백질을 파괴하고 코딩 변경을 포함하는 BRCA1 포인트 돌연변로 대체하여 DNA 손상의 동종 재조합의 수리를 위해 조직 문화를 기반으로 분석에서 BRCA1 변종을 테스트하기위한 방법을 제공합니다.
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