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DOI :
10.3791/2484-v
•
9:52 min
February 8th, 2011
Chapters
0:05
Title
1:32
Mechanical and Enzymatic Salivary Gland Tissue Digestion
5:00
Preparation of Salivary Gland Tissue for Plating
7:32
Plating Salivary Gland Cells
8:39
Examination of Salispheres and Proliferating Cells Using Phase-Contrast Microscopy and Immunostaining
9:34
Conclusion
マウス唾液腺由来の幹細胞の単離のため最適化されたプロトコルが記述されています。方法は、酵素と機械的な消化を採用しており、幹細胞の特性を持つ細胞を含むsalispheresの分離を可能にします。
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