March 11th, 2011
•A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.
Tags
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved