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DOI :
10.3791/2498-v
•
11:19 min
February 25th, 2011
Chapters
0:05
Title
2:59
Preparation of Cell Free Extracts Depleted of RF2
4:28
Preparation of a DNA Template
5:18
Preparation of Biotin Labeled mRNA
6:19
Isolation of Translating Ribosomes Containing a Peptidyl-tRNA
7:56
Analysis of the Isolated Ribosomes Containing the Peptidyl-tRNA
9:23
Representative Results
10:52
Conclusion
含新生肽- tRNA的核糖体的生化分析的一个主要障碍已被其他核糖体的存在,在同一样品的核糖体不参与翻译的特定基因序列分析。我们制定了一个简单的方法,净化,完全包含利益的新生肽酰- tRNA,核糖体。
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