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DOI :
10.3791/2498-v
•
11:19 min
February 25th, 2011
Chapters
0:05
Title
2:59
Preparation of Cell Free Extracts Depleted of RF2
4:28
Preparation of a DNA Template
5:18
Preparation of Biotin Labeled mRNA
6:19
Isolation of Translating Ribosomes Containing a Peptidyl-tRNA
7:56
Analysis of the Isolated Ribosomes Containing the Peptidyl-tRNA
9:23
Representative Results
10:52
Conclusion
ペプチジル- tRNAの初期段階を含むリボソームの生化学的解析への大きな障害は、同じサンプル中の他のリボソーム、解析対象の特定のmRNA配列の翻訳に関与していないリボソームの存在となっています。我々は、排他的に、興味の発生期のペプチジル- tRNAを含むリボソームを精製するための単純な方法論を開発。
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