Name: a microfluidic-based hydrodynamic trap for single particles
Uploaded: 2011-01-21T17:30:00
Description: Watch this Scientific Journal Video about A Microfluidic-based Hydrodynamic Trap for Single Particles at JoVE.com

我们感谢Kenis集团在伊利诺伊大学厄巴纳 - 香槟分校的有益讨论，并慷慨地提供洁净室设施的使用。 这项工作是由美国国立卫生研究院的途径，以独立的PI奖，根据批准号4R00HG004183 - 03（查尔斯M施罗德和Melikhan Tanyeri）。 这项工作是支持由美国国家科学基金会通过一个研究生研究奖学金埃里克M.约翰逊查瓦里亚。

由两层混合（聚二甲基硅氧烷（PDMS）/玻璃）粒子约束微流体装置的水动力的陷阱。步骤1-2描述的微流体装置的制造和步骤3-4讨论设备的设计和操作。 1。 SU - 8模具制作（视频没有显示） <ol><li>用丙酮和异丙醇（IPA）清洁两个硅片（直径3“）。 </li><li>与N 2，放在烤盘上在65℃1分钟以去除残留的水分干晶圆。 </li><li>自旋Coat晶圆＃1 SU - 8 2050光阻（PR），在4000转30秒创?...

<ol>
	<li>Tanyeri, M., Johnson-Chavarria, E. M., Schroeder, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrodynamic+Trap+for+Single+Particles+and+Cells.">Hydrodynamic Trap for Single Particles and Cells.</a> Applied Physics Letters. 96, 224101-224101 (2010).</li><li>Ashkin, A., Dziedzic, J. M., Bjorkholm, J. E., Chu, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Observation+of+a+Single-Beam+Gradient+Force+Optical+Trap+for+Dielectric+Particles.">Observation of a Single-Beam Gradient Force Optical Trap for Dielectric Particles.</a> Optics Letters. 11, 288-290 (1986).</li><li>Neuman, K. C., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping.">Optical trapping.</a> Review of Scientific Instruments. 75, 2787-2809 (2004).</li><li>Gosse, C., Croquette, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+tweezers:+Micromanipulation+and+force+measurement+at+the+molecular+level.">Magnetic tweezers: Micromanipulation and force measurement at the molecular level.</a> Biophysical Journal. 82, 3314-3329 (2002).</li><li>Chiou, P. Y., Ohta, A. T., Wu, M. C., C, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Massively+parallel+manipulation+of+single+cells+and+microparticles+using+optical+images.">Massively parallel manipulation of single cells and microparticles using optical images.</a> Nature. 436, 370-372 (2005).</li><li>Cohen, A. E., Moerner, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Method+for+trapping+and+manipulating+nanoscale+objects+in+solution.">Method for trapping and manipulating nanoscale objects in solution.</a> Applied Physics Letters. 86, 093109-09 (2005).</li><li>Evander, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+acoustic+cell+trapping+in+a+microfluidic+perfusion+system+for+online+bioassays&amp;quot;,+Analytical+Chemistry+79.">Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays&amp;quot;, Analytical Chemistry 79.</a> , 2984-2991 (2007).</li><li>Unger, M. A., Chou, H. P., Thorsen, T., Scherer, A., Quake, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monolithic+microfabricated+valves+and+pumps+by+multilayer+soft+lithography.">Monolithic microfabricated valves and pumps by multilayer soft lithography.</a> Science. 288, 113-116 (2000).</li><li>Kim, M. C., Wang, Z. H., Lam, R. H. W., Thorsen, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Building+a+better+cell+trap:+Applying+Lagrangian+modeling+to+the+design+of+microfluidic+devices+for+cell+biology.">Building a better cell trap: Applying Lagrangian modeling to the design of microfluidic devices for cell biology.</a> Journal of Applied Physics. 103, (2008).</li><li>Lutz, B. R., Chen, J., Schwartz, D. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrodynamic+tweezers:+1.+Noncontact+trapping+of+single+cells+using+steady+streaming+microeddies.">Hydrodynamic tweezers: 1. Noncontact trapping of single cells using steady streaming microeddies.</a> Analytical Chemistry. 78, 5429-5435 (2006).</li></ol>

目前基于流体力学流动的粒子操纵微流体的方法可以被定性为接触式或非接触的方法。接触式的方法使用流体流动的物理限制和固定颗粒对微通道的墙壁 9，非接触式方法，而依靠循环流量或microeddies 10 。在这项工作中，我们提出一个自由的解决方案颗粒流体流动的唯一行动诱捕方法。在一个微十字槽设备的流体驻点流体力学的陷阱，使小颗粒的约束和操纵。在这种设备中，自动?...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>21 gauge blunt needle</td>
<td>&nbsp;</td>
<td> Zephyrtronics</td>
<td> ZT-5-021-1-L</td>
<td>For punching port holes in PDMS</td>
</tr>
<tr>
<td>3 ml plastic syringe</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309585</td>
<td>For filling valve with oil</td>
</tr>
<tr>
<td>Si wafers</td>
<td>&nbsp;</td>
<td>University Wafer</td>
<td>&nbsp;</td>
<td> 3&#x201D; P(100) single side polished 380 &mu;m test grade</td>
</tr>
<tr>
<td>Cover glass</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>48404-428</td>
<td>24 x 40 mm #1.5</td>
</tr>
<tr>
<td>DAQ card</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>PCI 6229</td>
<td>&nbsp;</td>
<tr>
<td>Fluorescent beads</td>
<td>&nbsp;</td>
<td>Spherotech</td>
<td>FP-2056-2</td>
<td>2.2 &mu;m Nile red</td>
</tr>
<tr>
<td>Fluorinert </td>
<td>&nbsp;</td>
<td>3M</td>
<td>FC 40</td>
<td>Fluorinated carrier oil</td>
</tr>
<tr>
<td>Inverted Microscope</td>
<td>&nbsp;</td>
<td>Olympus</td>
<td>IX-71</td>
<td>&nbsp;</td>
<tr>
<td>LabVIEW</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>Version 9.0f3 (32bit)</td>
<td>&nbsp;</td>
<tr>
<td>Stereo Microscope</td>
<td>&nbsp;</td>
<td>Leica</td>
<td>MZ6</td>
<td>For aligning PDMS control layer to fluidic layer.</td>
</tr>
<tr>
<td>Mechanical Convection Oven</td>
<td>&nbsp;</td>
<td> VWR</td>
<td>1300U</td>
<td>For baking devices to create monolithic PDMS slabs with two layers.</td>
</tr>
<tr>
<td>Microfluidic tubing and connectors</td>
<td>&nbsp;</td>
<td>Upchurch Scientific</td>
<td>&nbsp;</td>
<td>1/16 x .020 PFA tubing and super flangeless fittings</td>
</tr>
<tr>
<td>PDMS</td>
<td>&nbsp;</td>
<td>GE Silicones</td>
<td>RTV 615 A&amp;B</td>
<td>&nbsp;</td>
<tr>
<td>Plasma Chamber</td>
<td>&nbsp;</td>
<td>Harrick</td>
<td>PDC-001</td>
<td>&nbsp;</td>
<tr>
<td>Pressure Transducer</td>
<td>&nbsp;</td>
<td>Proportion Air</td>
<td>DQPV1</td>
<td>&nbsp;</td>
<tr>
<td>Spin Coater</td>
<td>&nbsp;</td>
<td>Specialty Coating Systems</td>
<td>G3P-8 Spin Coat</td>
<td>&nbsp;</td>
<tr>
<td>Photoresist</td>
<td>&nbsp;</td>
<td>MicroChem</td>
<td>SU 8 2050</td>
<td>&nbsp;</td>
<tr>
<td>Syringe Pump</td>
<td>&nbsp;</td>
<td>Harvard Apparatus</td>
<td>PHD 2000 Programmable</td>
<td>&nbsp;</td>
<tr>
<td>Terminal Block</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>BNC 2110</td>
<td>For analog output to pressure regulator and read out.</td>
</tr>
<tr>
<td>UV Collimated Light Source and Exposure System</td>
<td>&nbsp;</td>
<td>OAI</td>
<td>Model 30 Enhanced Light Source</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

a microfluidic-based hydrodynamic trap for single particles

限制和操纵单个粒子在免费的解决方案的能力是一个关键的有利基础和​​应用科学技术。导致在物理学和生物学的重大进展，包括从分子细胞水平上的光，磁，电动，声等技术为基础的粒子诱捕方法。在这篇文章中，我们将介绍一个基于新的微流体技术，完全基于流体力学流体流动的颗粒捕集和操纵。使用这种方法，我们展示了诱捕的微观和纳米尺度粒子在水溶液中长时间尺度。水力陷阱，包括一个综合的跨槽通道的几何形状，两个对立的层流的融合，从而产生了一种平面流体停滞点（零速度点）伸展流动的微流体装置。在这种设备中，粒子只限于在流场主动控制的陷阱中心，保持在流体驻点粒子的位置。在这种方式下，粒子有效地被困在免费的解决方案，使用一个定制的LabVIEW代码实现反馈控制算法。控制算法由一个粒子在微流体装置，粒子跟踪，颗粒质心位置的决心，积极调整，通过调节应用到一个芯片上的气动阀压力调节器的压力和流体流动的图像采集。这样，芯片上的动态计量阀功能调节的出口渠道相对流速，从而使停滞点的位置和颗粒捕集细规模控制。基于微流体的流体力学陷阱展品颗粒捕集方法的几个优点。未经被困对象的物理或化学性质的具体要求任意粒子的流体力学诱捕。此外，水动力诱捕可以集中或拥挤的粒子悬浮在“单一”的目标对象，这是很难用替代的力量基于现场的诱捕方法的约束。水力陷阱是用户友好，直接实现，并可能被添加到现有的微流体装置，以方便长时间粒子的捕获和分析。总体而言，水动力陷阱是一个新的平台，和无颗粒表面固定的禁闭，显微观察和消除潜在的微扰光，磁，电领域的解决方案的小颗粒的自由诱捕的需要。

Watch this Scientific Journal Video about A Microfluidic-based Hydrodynamic Trap for Single Particles at JoVE.com

A Microfluidic-based Hydrodynamic Trap for Single Particles

在这篇文章中，我们提出一个基于微流体的方法，根据流体力学流动的粒子约束。我们证明使用的反馈控制机制，在流体停滞点稳定的粒子诱捕，从而使分娩和任意颗粒显微集成微装置。

一个单粒子为基础的微流控液力陷阱

The ability to confine and manipulate single particles in free solution is a key enabling technology for fundamental and applied science.  Methods for ...

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