Name: a microfluidic-based hydrodynamic trap for single particles
Uploaded: 2011-01-21T17:30:00
Description: Watch this Scientific Journal Video about A Microfluidic-based Hydrodynamic Trap for Single Particles at JoVE.com

हम उपयोगी विचार - विमर्श के लिए Urbana-Champaign में इलिनोइस विश्वविद्यालय में Kenis समूह धन्यवाद और उदारता cleanroom सुविधाओं का उपयोग प्रदान. यह काम एक NIH मार्ग से आजादी PI पुरस्कार के लिए अनुदान सं 4R00HG004183 03-(चार्ल्स एम. श्रोएडर और Melikhan Tanyeri) के तहत वित्त पोषित किया गया था,. इस काम एरिक जॉनसन Chavarria एम. एक ग्रेजुएट रिसर्च फैलोशिप के माध्यम से राष्ट्रीय विज्ञान फाउंडेशन द्वारा समर्थित किया गया था.

hydrodynamic जाल एक दो परत संकर (polydimethylsiloxane (PDMS) / कांच) कण प्रसूति के लिए microfluidic डिवाइस के होते हैं. 1-2 कदम microfluidic उपकरणों के निर्माण का वर्णन है, और 3-4 पर चर्चा डिवाइस डिजाइन और ऑपरेशन कदम. 1. SU-8 मोल्ड निर्माण (वीडियो ...

<ol>
	<li>Tanyeri, M., Johnson-Chavarria, E. M., Schroeder, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrodynamic+Trap+for+Single+Particles+and+Cells.">Hydrodynamic Trap for Single Particles and Cells.</a> Applied Physics Letters. 96, 224101-224101 (2010).</li><li>Ashkin, A., Dziedzic, J. M., Bjorkholm, J. E., Chu, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Observation+of+a+Single-Beam+Gradient+Force+Optical+Trap+for+Dielectric+Particles.">Observation of a Single-Beam Gradient Force Optical Trap for Dielectric Particles.</a> Optics Letters. 11, 288-290 (1986).</li><li>Neuman, K. C., Block, S. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+trapping.">Optical trapping.</a> Review of Scientific Instruments. 75, 2787-2809 (2004).</li><li>Gosse, C., Croquette, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+tweezers:+Micromanipulation+and+force+measurement+at+the+molecular+level.">Magnetic tweezers: Micromanipulation and force measurement at the molecular level.</a> Biophysical Journal. 82, 3314-3329 (2002).</li><li>Chiou, P. Y., Ohta, A. T., Wu, M. C., C, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Massively+parallel+manipulation+of+single+cells+and+microparticles+using+optical+images.">Massively parallel manipulation of single cells and microparticles using optical images.</a> Nature. 436, 370-372 (2005).</li><li>Cohen, A. E., Moerner, W. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Method+for+trapping+and+manipulating+nanoscale+objects+in+solution.">Method for trapping and manipulating nanoscale objects in solution.</a> Applied Physics Letters. 86, 093109-09 (2005).</li><li>Evander, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+acoustic+cell+trapping+in+a+microfluidic+perfusion+system+for+online+bioassays&amp;quot;,+Analytical+Chemistry+79.">Noninvasive acoustic cell trapping in a microfluidic perfusion system for online bioassays&amp;quot;, Analytical Chemistry 79.</a> , 2984-2991 (2007).</li><li>Unger, M. A., Chou, H. P., Thorsen, T., Scherer, A., Quake, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monolithic+microfabricated+valves+and+pumps+by+multilayer+soft+lithography.">Monolithic microfabricated valves and pumps by multilayer soft lithography.</a> Science. 288, 113-116 (2000).</li><li>Kim, M. C., Wang, Z. H., Lam, R. H. W., Thorsen, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Building+a+better+cell+trap:+Applying+Lagrangian+modeling+to+the+design+of+microfluidic+devices+for+cell+biology.">Building a better cell trap: Applying Lagrangian modeling to the design of microfluidic devices for cell biology.</a> Journal of Applied Physics. 103, (2008).</li><li>Lutz, B. R., Chen, J., Schwartz, D. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hydrodynamic+tweezers:+1.+Noncontact+trapping+of+single+cells+using+steady+streaming+microeddies.">Hydrodynamic tweezers: 1. Noncontact trapping of single cells using steady streaming microeddies.</a> Analytical Chemistry. 78, 5429-5435 (2006).</li></ol>

कण हेरफेर के लिए hydrodynamic प्रवाह के आधार पर वर्तमान microfluidic तरीकों संपर्क आधारित या गैर संपर्क तरीके के रूप में लक्षण वर्णन किया जा सकता है. संपर्क - आधारित विधियों तरल प्रवाह का उपयोग करने के लिए शारीरिक रूप <su...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
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<tr>
<td>21 gauge blunt needle</td>
<td>&nbsp;</td>
<td> Zephyrtronics</td>
<td> ZT-5-021-1-L</td>
<td>For punching port holes in PDMS</td>
</tr>
<tr>
<td>3 ml plastic syringe</td>
<td>&nbsp;</td>
<td>BD</td>
<td>309585</td>
<td>For filling valve with oil</td>
</tr>
<tr>
<td>Si wafers</td>
<td>&nbsp;</td>
<td>University Wafer</td>
<td>&nbsp;</td>
<td> 3&#x201D; P(100) single side polished 380 &mu;m test grade</td>
</tr>
<tr>
<td>Cover glass</td>
<td>&nbsp;</td>
<td>VWR</td>
<td>48404-428</td>
<td>24 x 40 mm #1.5</td>
</tr>
<tr>
<td>DAQ card</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>PCI 6229</td>
<td>&nbsp;</td>
<tr>
<td>Fluorescent beads</td>
<td>&nbsp;</td>
<td>Spherotech</td>
<td>FP-2056-2</td>
<td>2.2 &mu;m Nile red</td>
</tr>
<tr>
<td>Fluorinert </td>
<td>&nbsp;</td>
<td>3M</td>
<td>FC 40</td>
<td>Fluorinated carrier oil</td>
</tr>
<tr>
<td>Inverted Microscope</td>
<td>&nbsp;</td>
<td>Olympus</td>
<td>IX-71</td>
<td>&nbsp;</td>
<tr>
<td>LabVIEW</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>Version 9.0f3 (32bit)</td>
<td>&nbsp;</td>
<tr>
<td>Stereo Microscope</td>
<td>&nbsp;</td>
<td>Leica</td>
<td>MZ6</td>
<td>For aligning PDMS control layer to fluidic layer.</td>
</tr>
<tr>
<td>Mechanical Convection Oven</td>
<td>&nbsp;</td>
<td> VWR</td>
<td>1300U</td>
<td>For baking devices to create monolithic PDMS slabs with two layers.</td>
</tr>
<tr>
<td>Microfluidic tubing and connectors</td>
<td>&nbsp;</td>
<td>Upchurch Scientific</td>
<td>&nbsp;</td>
<td>1/16 x .020 PFA tubing and super flangeless fittings</td>
</tr>
<tr>
<td>PDMS</td>
<td>&nbsp;</td>
<td>GE Silicones</td>
<td>RTV 615 A&amp;B</td>
<td>&nbsp;</td>
<tr>
<td>Plasma Chamber</td>
<td>&nbsp;</td>
<td>Harrick</td>
<td>PDC-001</td>
<td>&nbsp;</td>
<tr>
<td>Pressure Transducer</td>
<td>&nbsp;</td>
<td>Proportion Air</td>
<td>DQPV1</td>
<td>&nbsp;</td>
<tr>
<td>Spin Coater</td>
<td>&nbsp;</td>
<td>Specialty Coating Systems</td>
<td>G3P-8 Spin Coat</td>
<td>&nbsp;</td>
<tr>
<td>Photoresist</td>
<td>&nbsp;</td>
<td>MicroChem</td>
<td>SU 8 2050</td>
<td>&nbsp;</td>
<tr>
<td>Syringe Pump</td>
<td>&nbsp;</td>
<td>Harvard Apparatus</td>
<td>PHD 2000 Programmable</td>
<td>&nbsp;</td>
<tr>
<td>Terminal Block</td>
<td>&nbsp;</td>
<td>National Instruments</td>
<td>BNC 2110</td>
<td>For analog output to pressure regulator and read out.</td>
</tr>
<tr>
<td>UV Collimated Light Source and Exposure System</td>
<td>&nbsp;</td>
<td>OAI</td>
<td>Model 30 Enhanced Light Source</td>
<td>&nbsp;</td>		</tbody>
		</table>
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a microfluidic-based hydrodynamic trap for single particles

The ability to confine and manipulate single particles in free solution is a key enabling technology for fundamental and applied science. Methods for particle trapping based on optical, magnetic, electrokinetic, and acoustic techniques have led to major advancements in physics and biology ranging from the molecular to cellular level. In this article, we introduce a new microfluidic-based technique for particle trapping and manipulation based solely on hydrodynamic fluid flow. Using this method, we demonstrate trapping of micro- and nano-scale particles in aqueous solutions for long time scales. The hydrodynamic trap consists of an integrated microfluidic device with a cross-slot channel geometry where two opposing laminar streams converge, thereby generating a planar extensional flow with a fluid stagnation point (zero-velocity point). In this device, particles are confined at the trap center by active control of the flow field to maintain particle position at the fluid stagnation point. In this manner, particles are effectively trapped in free solution using a feedback control algorithm implemented with a custom-built LabVIEW code. The control algorithm consists of image acquisition for a particle in the microfluidic device, followed by particle tracking, determination of particle centroid position, and active adjustment of fluid flow by regulating the pressure applied to an on-chip pneumatic valve using a pressure regulator. In this way, the on-chip dynamic metering valve functions to regulate the relative flow rates in the outlet channels, thereby enabling fine-scale control of stagnation point position and particle trapping. The microfluidic-based hydrodynamic trap exhibits several advantages as a method for particle trapping. Hydrodynamic trapping is possible for any arbitrary particle without specific requirements on the physical or chemical properties of the trapped object. In addition, hydrodynamic trapping enables confinement of a "single" target object in concentrated or crowded particle suspensions, which is difficult using alternative force field-based trapping methods. The hydrodynamic trap is user-friendly, straightforward to implement and may be added to existing microfluidic devices to facilitate trapping and long-time analysis of particles. Overall, the hydrodynamic trap is a new platform for confinement, micromanipulation, and observation of particles without surface immobilization and eliminates the need for potentially perturbative optical, magnetic, and electric fields in the free-solution trapping of small particles.

Watch this Scientific Journal Video about A Microfluidic-based Hydrodynamic Trap for Single Particles at JoVE.com

A Microfluidic-based Hydrodynamic Trap for Single Particles

इस अनुच्छेद में, हम कण hydrodynamic प्रवाह पर आधारित प्रसूति के लिए एक microfluidic आधारित विधि प्रस्तुत करते हैं. हम एक प्रतिक्रिया नियंत्रण तंत्र का उपयोग कर एक तरल पदार्थ ठहराव बिंदु पर स्थिर कण फँसाने का प्रदर्शन, जिससे एक एकीकृत microdevice में सक्षम कारावास और मनमाने ढंग से कणों का micromanipulation.

एक microfluidic आधारित एकल कण के लिए द्रव्यगतिकी जाल

The ability to confine and manipulate single particles in free solution is a key enabling technology for fundamental and applied science.  Methods for ...

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