Name: mouse bladder wall injection
Uploaded: 2011-07-12T12:00:00
Description: Watch this Scientific Journal Video about Mouse Bladder Wall Injection at JoVE.com

我们非常感谢支持从斯坦福大学的儿科研究基金和K08DK087895 - 01的NIDDK试点授予。

1。鼠标膀胱壁注射液小鼠品系，年龄和性别的选择是出于实验需要。我们用8至12周龄小鼠，因为这是一个窗口前的免疫成熟到衰老。作为一般指引，老鼠应该到达至少一个星期前，实验操作，以避免应力引起的混杂因素。 <ol><li>用肥皂和水清洁手术台面。 </li><li> CIDE挥笔或杀菌湿巾擦拭手术台面。 </li><li>前高压灭菌干净的手术器械，手术中使用。 </li><li>此外，热珠灭菌消...

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	<li>Dinney, C. P., Fishbeck, R., Singh, R. K., Eve, B., Pathak, S., Brown, N., Xie, B., Fan, D., Bucana, C., Fidler, I. J., Killion, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+Characterization+of+Metastatic+Variants+from+Human+Transitional+Cell+Carcinoma+Passaged+by+Orthotopic+Implantation+in+Athymic+Nude+Mice.">Isolation and Characterization of Metastatic Variants from Human Transitional Cell Carcinoma Passaged by Orthotopic Implantation in Athymic Nude Mice.</a> The Journal of Urology. 154, 1532-1538 (1995).</li><li>Singh, A. V., Franke, A. A., Blackburn, G. 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小鼠膀胱壁注射允许为特定的细胞植入到膀胱壁的定义领域。这项技术已广泛应用，为膀胱癌，平滑肌，和干细胞生物学的小鼠模型。根据定义，小鼠膀胱壁注射液有利于引入膀胱癌或干细胞原位位置，所以可以在一个有关生理解剖背景下的研究，他们的生长和分化。事实上，Dinney等。首次描述了这种技术，以研究在1裸鼠人膀胱癌裸鼠移植瘤原位。 虽然一直在膀胱?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>异氟醚（Aerrane） </td><td>巴克斯特</td><td> NDC的10019-773-60 </td></tr><tr><td> 29或30表针</td><td>汉密尔顿</td><td> 7803-06或7803-07 </td></tr><tr><td> 100微升注射器</td><td>汉密尔顿</td><td> 7656-01 </td></tr></tbody></table>

mouse bladder wall injection

小鼠膀胱壁注射原位研究，包括干细胞，平滑肌，和癌症生物学膀胱现象，是一种有用的技术。开始前注射，手术区必须用肥皂和水和消毒液清洗。在使用前和每个动物之间的手术设备必须消毒。每个鼠标放在吸入异氟醚麻醉下（2-5％的诱导，维持1-3％）和膀胱暴露中线腹部切口，用剪刀。如果膀胱满了，这是部分之间的两根手指轻轻挤压解压。 intramurally利益的细胞悬液注入使用29或30号针头和注射器1毫升或更小的圆顶膀胱壁。伤口闭合伤口剪辑，并允许恢复变暖垫鼠标。膀胱壁注射液是一种微妙的显微外科技术，可以掌握与实践。

Watch this Scientific Journal Video about Mouse Bladder Wall Injection at JoVE.com

Mouse Bladder Wall Injection

小鼠膀胱壁注射液是一种有用的方法原位研究膀胱干细胞和癌症生物学。这种微妙的显微外科方法可以掌握仔细的技术和实践。

鼠标膀胱壁注射液

Mouse bladder wall injection is a useful technique to orthotopically study bladder phenomena, including stem cell, smooth muscle, and cancer biology. ...

Research

JoVE Journal

Medicine

An Orthotopic Model of Murine Bladder Cancer

一个小鼠膀胱癌原位模型

In this straightforward procedure, bladder tumors are established in female C57 mice through the use of catheterization, local cauterization, and ...

科研

医学

The Use of Cystometry in Small Rodents: A Study of Bladder Chemosensation

的的膀胱测压小老鼠：A膀胱Chemosensation研究的使用

	The lower urinary tract (LUT) functions as a  dynamic reservoir that is able to store urine and to efficiently expel it at a  convenient time. While ...

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-&kappa;B Reporter Transgenic

Intraductal Injection of LPS as a Mouse Model of Mastitis: Signaling Visualized via an NF-ÃƒÅ½Ã‚ÂºB Reporter Transgenic

导管内可视化通过NF-κB记者的转基因小鼠模型的乳腺炎：信号注射LPS

Animal models of human disease are necessary in order to rigorously study stages of disease progression and associated mechanisms, and ultimately, as ...

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

原位膀胱肿瘤模型和评价治疗膀胱萨纳

We present a novel method for treating bladder cancer with intravesically delivered small activating RNA (saRNA) in an orthotopic xenograft mouse bladder ...

Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye

基因治疗的载体和干细胞视网膜下注射到老鼠的眼睛

The loss of sight affects approximately 3.4 million people in the United States and is expected to increase in the upcoming years.1 Recently, gene therapy ...

Reduction in Left Ventricular Wall Stress and Improvement in Function in Failing Hearts using Algisyl-LVR

左室壁应力和改进功能衰竭心脏使用Algisyl LVR减少

Injection of  Algisyl-LVR, a treatment under clinical development, is intended to treat  patients with dilated cardiomyopathy. This treatment was recently ...

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

原位膀胱癌模型的基因传递研究

Bladder cancer is the second most common cancer of the urogenital tract and novel therapeutic approaches that can reduce recurrence and progression are ...

Minimally Invasive Establishment of Murine Orthotopic Bladder Xenografts

微创建立小鼠原位膀胱移植瘤的

Orthotopic bladder cancer xenografts are the gold standard to study molecular cellular manipulations and new therapeutic agents&#160;in vivo. Suitable ...

Ultrasound Imaging-guided Intracardiac Injection to Develop a Mouse Model of Breast Cancer Brain Metastases Followed by Longitudinal MRI

超声成像引导下腔内注射到患乳腺癌脑转移其次是纵向的MRI的小鼠模型

Breast cancer brain metastasis, occurring in 30% of breast cancer patients at stage IV, is associated with high mortality. The median survival is only 6 ...

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

膀胱胀诱发内脏运动反应为膀胱疼痛小鼠模型

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition ...