Name: use of a hanging weight system for coronary artery occlusion in mice
Uploaded: 2011-04-19T11:00:00
Duration: 8 min 30 s
Description: Watch this Scientific Journal Video about Use of a Hanging Weight System for Coronary Artery Occlusion in Mice at JoVE.com

<p class="jove_content"> वर्तमान अध्ययन राष्ट्रीय हृदय, फेफड़े और रक्त संस्थान R01 HL0921 अनुदान, R01-DK083385 और एच Eltzschig R01-HL098294, टी. Eckle 1K08HL102267-01, और संज्ञाहरण शिक्षा और टी. करने के लिए अनुसंधान अनुदान के लिए फाउंडेशन द्वारा समर्थित हैं Eckle और एच Eltzschig, और अमेरिकन हार्ट एसोसिएशन टी. Eckle और एच Eltzschig और ड्यूश Forschungsgemeinschaft (DFG) एम. Koeppen अनुसंधान के लिए फैलोशिप के लिए अनुदान. हम कला का काम शेली Eltzschig के लिए धन्यवाद. </p>

ब्याज की कोई संघर्ष की घोषणा की.

<p class="jove_content"> वर्तमान अध्ययन एक अक्षुण्ण murine मॉडल फांसी वजन एक प्रणाली का उपयोग में आईपी प्रदर्शन और इस तरह एक गाँठ द्वारा कोरोनरी धमनी रोड़ा से बचने का एक उपन्यास तकनीक का वर्णन करता है. वास्तव में, इस अध्ययन आईपी द्वारा अत्यधिक प्रतिलिपि प्रस्तुत करने योग्य रोधगलितांश आकार और हृदय संरक्षण को दर्शाता है, इस प्रकार गाँठ आधारित कोरोनरी रोड़ा मॉडलों के साथ जुड़े परिवर्तनशीलता कम से कम. जो चूहों में आईपी द्वारा cardioprotection का अध्ययन पर विचार जांचकर्ता इस मॉडल से लाभ हो सकता है. </p>

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use of a hanging weight system for coronary artery occlusion in mice

<p class="jove_content">Murine studies of acute injury are an area of intense investigation, as knockout mice for different genes are becoming increasingly available <sup>1-38</sup>. Cardioprotection by ischemic preconditioning (IP) remains an area of intense investigation. To further elucidate its molecular basis, the use of knockout mouse studies is particularly important <sup>7, 14, 30, 39</sup>. Despite the fact that previous studies have already successfully performed cardiac ischemia and reperfusion in mice, this model is technically very challenging. Particularly, visual identification of the coronary artery, placement of the suture around the vessel and coronary occlusion by tying off the vessel with a supported knot is technically difficult. In addition, re-opening the knot for intermittent reperfusion of the coronary artery during IP without causing surgical trauma adds additional challenge. Moreover, if the knot is not tied down strong enough, inadvertent reperfusion due to imperfect occlusion of the coronary may affect the results. In fact, this can easily occur due to the movement of the beating heart.</p>
<p class="jove_content">Based on potential problems associated with using a knotted coronary occlusion system, we adopted a previously published model of chronic cardiomyopathy based on a hanging weight system for intermittent coronary artery occlusion during IP <sup>39</sup>. In fact, coronary artery occlusion can thus be achieved without having to occlude the coronary by a knot. Moreover, reperfusion of the vessel can be easily achieved by supporting the hanging weights which are in a remote localization from cardiac tissues.</p>
<p class="jove_content">We tested this system systematically, including variation of ischemia and reperfusion times, preconditioning regiments, body temperature and genetic backgrounds<sup>39</sup>. In addition to infarct staining, we tested cardiac troponin I (cTnI)
as a marker of myocardial infarction in this model. In fact, plasma levels of cTnI correlated with infarct sizes (R2=0.8). Finally, we could show in several studies that this technique yields highly reproducible infarct sizes during murine IP and myocardial infarction<sup>6, 8, 30, 40, 41</sup>. Therefore, this technique may be helpful for researchers who pursue molecular mechanisms involved in cardioprotection by IP using a genetic approach in mice with targeted gene deletion. Further studies on cardiac IP using transgenic mice may consider this technique.</p>

Watch this Scientific Journal Video about Use of a Hanging Weight System for Coronary Artery Occlusion in Mice at JoVE.com

Use of a Hanging Weight System for Coronary Artery Occlusion in Mice

<p class="jove_content">Myocardial ischemia और इस्कीमिक शर्त के लिए एक murine मॉडल एक महत्वपूर्ण उपकरण अध्ययन cardioprotective तंत्र है<em&gt; Vivo में</em&gt;. यहाँ, हम एक आसान लागू रिपोर्ट<em&gt; बगल में</em&gt; हृदय कोरोनरी धमनी रोड़ा के लिए फांसी वजन एक प्रणाली का उपयोग कर आईपी के लिए मॉडल.</p>

चूहे में कोरोनरी धमनी आड़ के लिए एक हैंगिंग वजन सिस्टम का प्रयोग

Murine studies of acute injury are an area of intense investigation, as knockout mice for different genes are becoming increasingly available 1-38. ...

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