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DOI :
10.3791/2536-v
•
13:45 min
April 27th, 2011
Chapters
0:05
Title
1:00
Introduction
2:23
Nucleic Acid Extraction
3:45
Reverse Transcription and 2nd-strand cDNA Synthesis ("Round A")
5:36
PCR Amplification of Randomly Primed cDNA
6:40
Cy3 Fluorescent Dye Incorporation
8:02
Virochip Microarray Hybridization, Scanning and Analysis
10:34
Virus Detection Using Virochip Microarray
12:15
Conclusion
Virochipは同時に保存された配列の相同性に基づいてすべての既知のウイルスだけでなく、新たなウイルスを検出するように設計されたパンウイルスマイクロアレイです。ここでは、既知および未知のウイルスの存在のために臨床サンプルを解析するVirochipアッセイを実行する方法を示しています。
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