Alta MicroRNA profiling Throughput: Multiplex Ottimizzato qRT-PCR a scala nanoliter sul Fluidigm dinamica IFCS ArrayTM
August 3rd, 2011
•1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco , 2Center for Reproductive Sciences, University of California San Francisco , 3Department of Urology, University of California San Francisco , 4Department of Cell and Tissue Biology, University of California San Francisco , 5Fluidigm Corporation, Fluidigm Corporation , 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco
Qui descrivere un ottimizzato trascrittasi inversa multiplex PCR quantitativa (QRT-PCR) protocollo in combinazione con una piattaforma microfluidica come un efficace costi e tempi high-throughput screening per microRNA (miRNA) livelli di espressione, soprattutto quando si lavora con quantità limitate di campione.
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