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DOI :
10.3791/2555-v
April 22nd, 2011
Chapters
0:05
Title
1:15
Kidney Dissection and Capsule Removal
3:23
Enzymatic Digestion of Kidneys and Isolation of Nephrogenic Zone Cells
6:30
Phase Contrast and Fluorescent Microscopy of Purified Nephrogenic Zone Cells
7:24
Conclusion
本報告書では、発展途上腎臓の幹細胞/前駆細胞を調節するシグナル伝達経路を研究するために使用できるマウス胎児の腎臓から前駆細胞のニッチの分離と培養のための方法を説明します。これらの培養細胞は、候補の経路の効率的な操作を可能にする、小分子および組換えタンパク質の治療に、そして重要なことは、ウイルス形質導入に非常にアクセス可能です。
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