Name: assessing signaling properties of ectodermal epithelia during craniofacial development
Uploaded: 2011-03-24T11:00:00
Description: Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Dit werk werd gefinancierd door R01-R01-DE018234 en DE019638.

1. De voorbereiding van het donorweefsel <ol><li> Bereid cultuur media, scherp glas spelden, scherper wolfraam naalden. </li><li> Verzamel embryo van Shell, wassen in ijskoud PBS. <ol class="lalpha"><li> Met behulp van een 10 ml spuit en een 18 gauge naald te verwijderen 1,0 ml albumine uit het puntige uiteinde van de eierschaal. </li><li> Maak een klein gaatje aan de bovenzijde van het reservoir met behulp van de punt van de schaar, en sneed een ronde opening aan de embryo bloot </li></ol></li><li> Verwijder de kop e...

<ol>
	<li>Noden, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Role+of+the+Neural+Crest+in+Patterning+of+Avian+Cranial+Skeletal,+Connective,+and+Muscle+Tissues.">The Role of the Neural Crest in Patterning of Avian Cranial Skeletal, Connective, and Muscle Tissues.</a> Developmental Biology. 96, 144-144 (1983).</li><li>Bronner-Fraser, M., Stern, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Mesodermal+Tissues+on+Avian+Neural+Crest+Cell+Migration.">Effects of Mesodermal Tissues on Avian Neural Crest Cell Migration.</a> Developmental Biology. 143, 213-213 (1991).</li><li>Schneider, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+crest+can+form+cartilages+normally+derived+from+mesoderm+during+development+of+the+avian+head+skeleton.">Neural crest can form cartilages normally derived from mesoderm during development of the avian head skeleton.</a> Developmental Biology. 208, 441-441 (1999).</li><li>Couly, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interactions+between+Hox-negative+cephalic+neural+crest+cells+and+the+foregut+endoderm+in+patterning+the+facial+skeleton+in+the+vertebrate+head.">Interactions between Hox-negative cephalic neural crest cells and the foregut endoderm in patterning the facial skeleton in the vertebrate head.</a> Development. 129, 1061-1061 (2002).</li><li>Evans, D. J., Noden, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatial+relations+between+avian+craniofacial+neural+crest+and+paraxial+mesoderm+cells.">Spatial relations between avian craniofacial neural crest and paraxial mesoderm cells.</a> Dev Dyn. , (2006).</li><li>Hu, D., Marcucio, R., Helms, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+zone+of+frontonasal+ectoderm+regulates+patterning+and+growth+in+the+face.">A zone of frontonasal ectoderm regulates patterning and growth in the face.</a> Development. 130, 1749-1749 (2003).</li><li>Hu, D., Marcucio, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unique+organization+of+the+frontonasal+ectodermal+zone+in+birds+and+mammals.">Unique organization of the frontonasal ectodermal zone in birds and mammals.</a> Dev Biol. 325, 200-200 (2009).</li><li>Le Li&#232;vre, C. S., Le Douarin, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+derivatives+of+the+neural+crest:+analysis+of+chimaeric+quail+and+chick+embryos.">Mesenchymal derivatives of the neural crest: analysis of chimaeric quail and chick embryos.</a> Journal of Embryology and Experimental Morphology. 34, 125-125 (1975).</li><li>Bollag, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+a+repetitive+mouse+B2+element+to+identify+transplanted+mouse+cells+in+mouse-chick+chimeras.">Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras.</a> Exp Cell Res. 248, 75-75 (1999).</li><li>Korn, M. J., Cramer, K. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Windowing+chicken+eggs+for+developmental+studies.">Windowing chicken eggs for developmental studies.</a> J Vis Exp. , (2007).</li><li>Eames, B. F., Schneider, R. 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Met behulp van deze transplantatie methode heeft ons toegestaan ​​om te bepalen dat het ectoderm signalering informatie die dorsoventral polariteit en proximodistal uitbreiding van de bovenkaak regelt bevat. De gelijkenis van de uitkomsten bij het ​​gebruik van kwartel of de muis ectoderm, en het behoud van de moleculaire signalen in dit weefsel onder veel diersoorten 6,11 geeft aan dat dit een zeer goed geconserveerd signalering centrum onder de gewervelde dieren. Bovendien hebben andere onderzoekers...

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<td>1x PBS</td>
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<td>TEK</td>
<td>TEKZR114</td>
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<td>GIBCO</td>
<td>17105-041</td>
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<td>35x10 mm Petri dish</td>
<td>&nbsp;</td>
<td>Falcon</td>
<td>1008</td>
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<td>No. 5 Dumont forceps</td>
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<td>Fine Science Tools</td>
<td>11252-20</td>
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<td>Scissors</td>
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<td>Fine Science Tools</td>
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<td>Fine Science Tools</td>
<td>26000</td>
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<td>Microcapillary tube </td>
<td>&nbsp;</td>
<td>Drummond Scientific Company</td>
<td>3-000-225-G</td>
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<td>13-678-6B</td>
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<td>Spring scissors</td>
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<td>Fine Science Tools</td>
<td>15010-11 </td>
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<td>Blade holder</td>
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<td>Fine Science Tools</td>
<td>10052-11 </td>
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assessing signaling properties of ectodermal epithelia during craniofacial development

De toegankelijkheid van aviaire embryo&#39;s heeft geholpen experimentele embryologen begrijpen het lot van cellen tijdens de ontwikkeling en de rol van weefsel interacties die patronen en morfogenese van de gewervelde dieren (bijv. 1, 2, 3, 4) te regelen. Hier, illustreren we een methode die deze toegankelijkheid exploiteert aan de signalering en patroonvorming eigenschappen van de ectodermale weefsels te testen tijdens het gezicht ontwikkeling. In deze experimenten, maken we kwartel-chick 5 of muis-chick 6 hersenschimmen door het transplanteren van het oppervlak cefalische ectoderm dat de bovenkaak van de kwartel of de muis dekt op ofwel de dezelfde regio of een buitenbaarmoederlijke regio van kippenembryo&#39;s. Het gebruik van kwartels als donor weefsel voor transplantatie in kuikens werd ontwikkeld om te profiteren van een hergroepering van nucleoli marker aanwezig is in kwartel, maar niet kuiken cellen, waardoor de onderzoekers voor het hosten en de donor weefsels 7 te onderscheiden. Ook een repetitief element aanwezig is in de muis genoom en is alomtegenwoordig uitgedrukt, waardoor we gastheer en donor weefsels te onderscheiden in de muis-chick hersenschimmen 8. Het gebruik van de muis ectoderm als donor weefsel zal sterk uit te breiden ons begrip van deze weefsel interacties, want dit zal ons toelaten om de signalering eigenschappen van ectoderm ontleend aan diverse mutant embryo&#39;s te testen.

Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Dit artikel beschrijft een weefseltransplantatie techniek die is ontworpen om de signalering en patroonvorming eigenschappen van de oppervlakte Cephalic ectoderm tijdens de craniofaciale ontwikkeling test.

Het beoordelen van Signalering Eigenschappen van ectodermale epitheel Tijdens craniofaciale ontwikkeling

The accessibility of avian embryos has helped experimental embryologists understand the fates of cells during development and the role of tissue ...

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