Name: assessing signaling properties of ectodermal epithelia during craniofacial development
Uploaded: 2011-03-24T11:00:00
Description: Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Ce travail a été financé par R01 et R01-DE018234-DE019638.

1. Préparer le tissu du donneur <ol><li> Préparer les milieux de culture, d&#39;affiner broches verre, affûter les aiguilles de tungstène. </li><li> Recueillir l&#39;embryon de la coquille, lavez-les à PBS glacé. <ol class="lalpha"><li> En utilisant une seringue de 10 ml et une aiguille 18 gauge retirer 1,0 ml d&#39;albumine de l&#39;extrémité pointue de la coquille d&#39;oeuf. </li><li> Faire un petit trou sur le dessus de la coquille en utilisant le point de ciseaux, puis couper une ouverture circulaire pour...

<ol>
	<li>Noden, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Role+of+the+Neural+Crest+in+Patterning+of+Avian+Cranial+Skeletal,+Connective,+and+Muscle+Tissues.">The Role of the Neural Crest in Patterning of Avian Cranial Skeletal, Connective, and Muscle Tissues.</a> Developmental Biology. 96, 144-144 (1983).</li><li>Bronner-Fraser, M., Stern, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Mesodermal+Tissues+on+Avian+Neural+Crest+Cell+Migration.">Effects of Mesodermal Tissues on Avian Neural Crest Cell Migration.</a> Developmental Biology. 143, 213-213 (1991).</li><li>Schneider, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+crest+can+form+cartilages+normally+derived+from+mesoderm+during+development+of+the+avian+head+skeleton.">Neural crest can form cartilages normally derived from mesoderm during development of the avian head skeleton.</a> Developmental Biology. 208, 441-441 (1999).</li><li>Couly, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interactions+between+Hox-negative+cephalic+neural+crest+cells+and+the+foregut+endoderm+in+patterning+the+facial+skeleton+in+the+vertebrate+head.">Interactions between Hox-negative cephalic neural crest cells and the foregut endoderm in patterning the facial skeleton in the vertebrate head.</a> Development. 129, 1061-1061 (2002).</li><li>Evans, D. J., Noden, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spatial+relations+between+avian+craniofacial+neural+crest+and+paraxial+mesoderm+cells.">Spatial relations between avian craniofacial neural crest and paraxial mesoderm cells.</a> Dev Dyn. , (2006).</li><li>Hu, D., Marcucio, R., Helms, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+zone+of+frontonasal+ectoderm+regulates+patterning+and+growth+in+the+face.">A zone of frontonasal ectoderm regulates patterning and growth in the face.</a> Development. 130, 1749-1749 (2003).</li><li>Hu, D., Marcucio, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Unique+organization+of+the+frontonasal+ectodermal+zone+in+birds+and+mammals.">Unique organization of the frontonasal ectodermal zone in birds and mammals.</a> Dev Biol. 325, 200-200 (2009).</li><li>Le Li&#232;vre, C. S., Le Douarin, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+derivatives+of+the+neural+crest:+analysis+of+chimaeric+quail+and+chick+embryos.">Mesenchymal derivatives of the neural crest: analysis of chimaeric quail and chick embryos.</a> Journal of Embryology and Experimental Morphology. 34, 125-125 (1975).</li><li>Bollag, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+a+repetitive+mouse+B2+element+to+identify+transplanted+mouse+cells+in+mouse-chick+chimeras.">Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras.</a> Exp Cell Res. 248, 75-75 (1999).</li><li>Korn, M. J., Cramer, K. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Windowing+chicken+eggs+for+developmental+studies.">Windowing chicken eggs for developmental studies.</a> J Vis Exp. , (2007).</li><li>Eames, B. F., Schneider, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quail-duck+chimeras+reveal+spatiotemporal+plasticity+in+molecular+and+histogenic+programs+of+cranial+feather+development.">Quail-duck chimeras reveal spatiotemporal plasticity in molecular and histogenic programs of cranial feather development.</a> Development. 132, 1499-1499 (2005).</li><li>Odent, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+the+Sonic+hedgehog+(SHH+)+gene+during+early+human+development+and+phenotypic+expression+of+new+mutations+causing+holoprosencephaly.">Expression of the Sonic hedgehog (SHH ) gene during early human development and phenotypic expression of new mutations causing holoprosencephaly.</a> Hum Mol Genet. 8, 1683-1683 (1999).</li><li>Szabo-Rogers, H. 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En utilisant cette méthode de transplantation nous a permis de déterminer que l&#39;ectoderme contient des informations de signalisation qui régule la polarité dorso-ventrale et l&#39;extension proximodistal de la mâchoire supérieure. La similitude des résultats lors de l&#39;utilisation de caille ou ectoderme de la souris, et la conservation des signaux moléculaires dans ce tissu parmi les nombreuses espèces 6,11 indique qu&#39;il s&#39;agit d&#39;un centre de signalisation hautement conservée chez...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>1x PBS</td>
<td>&nbsp;</td>
<td>TEK</td>
<td>TEKZR114</td>
<td>&nbsp;</td>
<tr>
<td>DMEM</td>
<td>&nbsp;</td>
<td>UCSF</td>
<td>CCFDA003</td>
<td>&nbsp;</td>
<tr>
<td>BSA</td>
<td>&nbsp;</td>
<td>SIGMA</td>
<td>A7906</td>
<td>&nbsp;</td>
<tr>
<td>Dispase</td>
<td>&nbsp;</td>
<td>GIBCO</td>
<td>17105-041</td>
<td>&nbsp;</td>
<tr>
<td>35x10 mm Petri dish</td>
<td>&nbsp;</td>
<td>Falcon</td>
<td>1008</td>
<td>&nbsp;</td>
<tr>
<td>No. 5 Dumont forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11252-20</td>
<td>&nbsp;</td>
<tr>
<td>Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14058-11</td>
<td>&nbsp;</td>
<tr>
<td>Spring Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15010-11</td>
<td>&nbsp;</td>
<tr>
<td>Needle holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>26016-12</td>
<td>&nbsp;</td>
<tr>
<td>Tungsten Needle</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>26000</td>
<td>&nbsp;</td>
<tr>
<td>Microcapillary tube </td>
<td>&nbsp;</td>
<td>Drummond Scientific Company</td>
<td>3-000-225-G</td>
<td>&nbsp;</td>
<tr>
<td>Pasteur Pipets</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>13-678-6B</td>
<td>&nbsp;</td>
<tr>
<td>Spring scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15010-11 </td>
<td>&nbsp;</td>
<tr>
<td>Blade holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10052-11 </td>
<td>&nbsp;</td>
<tr>
<td>Razor blade</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10050-00</td>
<td>&nbsp;</td>		</tbody>
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assessing signaling properties of ectodermal epithelia during craniofacial development

L&#39;accessibilité des embryons aviaires a aidé à comprendre les embryologistes expérimentale du sort des cellules pendant le développement et le rôle des interactions tissus qui régulent la structuration et la morphogenèse des vertébrés (par exemple, 1, 2, 3, 4). Ici, nous illustrons une méthode qui exploite cette accessibilité pour tester les propriétés de signalisation et de structuration des tissus ectodermiques lors de l&#39;élaboration du visage. Dans ces expériences, nous créons de caille-chiches 5 ou 6 souris chimères chiches en transplantant l&#39;ectoderme de surface céphalique qui recouvre la mâchoire supérieure de caille ou de la souris sur soit la même région ou d&#39;une région extra-utérine d&#39;embryons de poulet. L&#39;utilisation de la caille en tissu du donneur pour une transplantation dans les poussins a été développé pour tirer parti d&#39;un marqueur nucléolaire présente dans les cellules de caille, mais pas chiches, permettant ainsi aux enquêteurs d&#39;hôte et de distinguer les tissus des donneurs 7. De même, un élément répétitif est présent dans le génome de la souris et est exprimée de façon ubiquitaire, ce qui nous permet de distinguer les tissus hôtes et les donateurs dans les souris chimères chiches 8. L&#39;utilisation de la souris comme ectoderme tissu du donneur va augmenter considérablement notre compréhension de ces interactions tissulaires, parce que cela va nous permettre de tester les propriétés de signalisation des issues de différentes ectoderme embryons mutants.

Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Cet article décrit une technique de transplantation de tissus qui a été conçu pour tester les propriétés de signalisation et de structuration de la surface ectoderme céphalique au cours du développement cranio-facial.

Évaluation des propriétés de signalisation ectodermique épithéliums au cours du développement cranio-facial

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