Name: assessing signaling properties of ectodermal epithelia during craniofacial development
Uploaded: 2011-03-24T11:00:00
Description: Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Diese Arbeit wurde von R01-R01-DE018234 und DE019638 finanziert.

1. Vorbereiten des Spendergewebe <ol><li> Bereiten Kulturmedien, schärfen Glas Stifte, schärfen Wolfram Nadeln. </li><li> Sammeln Embryo aus der Schale, in eiskaltem PBS waschen. <ol class="lalpha"><li> Mit einer 10 ml-Spritze und eine 18-Gauge-Nadel entfernen 1,0 ml Albumin aus dem spitzen Ende der Eierschale. </li><li> Machen Sie ein kleines Loch an der Oberseite des Gehäuses mit dem Punkt der Schere, und schneiden Sie dann eine kreisrunde Öffnung, um den Embryo aussetzen </li></ol></li><li> Entfernen Sie den Ko...

<ol>
	<li>Noden, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Role+of+the+Neural+Crest+in+Patterning+of+Avian+Cranial+Skeletal,+Connective,+and+Muscle+Tissues.">The Role of the Neural Crest in Patterning of Avian Cranial Skeletal, Connective, and Muscle Tissues.</a> Developmental Biology. 96, 144-144 (1983).</li><li>Bronner-Fraser, M., Stern, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Mesodermal+Tissues+on+Avian+Neural+Crest+Cell+Migration.">Effects of Mesodermal Tissues on Avian Neural Crest Cell Migration.</a> Developmental Biology. 143, 213-213 (1991).</li><li>Schneider, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neural+crest+can+form+cartilages+normally+derived+from+mesoderm+during+development+of+the+avian+head+skeleton.">Neural crest can form cartilages normally derived from mesoderm during development of the avian head skeleton.</a> Developmental Biology. 208, 441-441 (1999).</li><li>Couly, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Interactions+between+Hox-negative+cephalic+neural+crest+cells+and+the+foregut+endoderm+in+patterning+the+facial+skeleton+in+the+vertebrate+head.">Interactions between Hox-negative cephalic neural crest cells and the foregut endoderm in patterning the facial skeleton in the vertebrate head.</a> Development. 129, 1061-1061 (2002).</li><li>Evans, D. J., Noden, D. 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Mit dieser Transplantation Methode konnten wir feststellen, dass die Ektoderm Signalisierung Informationen, die dorsoventrale Polarität und proximodistalen Erweiterung des Oberkiefers regelt enthält. Die Ähnlichkeit der Ergebnisse bei der Verwendung von Wachteln oder Maus Ektoderm, und die Erhaltung der molekularen Signale in diesem Gewebe unter vielen Arten 6,11 bedeutet, dass eine hoch konservierte Signalisierung Zentrum unter den Wirbeltieren ist. Darüber hinaus haben andere Forscher ähnliche Technike...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<tr>
<td>1x PBS</td>
<td>&nbsp;</td>
<td>TEK</td>
<td>TEKZR114</td>
<td>&nbsp;</td>
<tr>
<td>DMEM</td>
<td>&nbsp;</td>
<td>UCSF</td>
<td>CCFDA003</td>
<td>&nbsp;</td>
<tr>
<td>BSA</td>
<td>&nbsp;</td>
<td>SIGMA</td>
<td>A7906</td>
<td>&nbsp;</td>
<tr>
<td>Dispase</td>
<td>&nbsp;</td>
<td>GIBCO</td>
<td>17105-041</td>
<td>&nbsp;</td>
<tr>
<td>35x10 mm Petri dish</td>
<td>&nbsp;</td>
<td>Falcon</td>
<td>1008</td>
<td>&nbsp;</td>
<tr>
<td>No. 5 Dumont forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>11252-20</td>
<td>&nbsp;</td>
<tr>
<td>Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>14058-11</td>
<td>&nbsp;</td>
<tr>
<td>Spring Scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15010-11</td>
<td>&nbsp;</td>
<tr>
<td>Needle holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>26016-12</td>
<td>&nbsp;</td>
<tr>
<td>Tungsten Needle</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>26000</td>
<td>&nbsp;</td>
<tr>
<td>Microcapillary tube </td>
<td>&nbsp;</td>
<td>Drummond Scientific Company</td>
<td>3-000-225-G</td>
<td>&nbsp;</td>
<tr>
<td>Pasteur Pipets</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>13-678-6B</td>
<td>&nbsp;</td>
<tr>
<td>Spring scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>15010-11 </td>
<td>&nbsp;</td>
<tr>
<td>Blade holder</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10052-11 </td>
<td>&nbsp;</td>
<tr>
<td>Razor blade</td>
<td>&nbsp;</td>
<td>Fine Science Tools</td>
<td>10050-00</td>
<td>&nbsp;</td>		</tbody>
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assessing signaling properties of ectodermal epithelia during craniofacial development

Die Zugänglichkeit der Vogelembryonen hat dazu beigetragen, experimentelle Embryologie verstehen das Schicksal der Zellen während der Entwicklung und der Rolle der Gewebe-Wechselwirkungen, die Strukturierung und Morphogenese von Wirbeltieren (z. B. 1, 2, 3, 4) zu regulieren. Hier zeigen wir eine Methode, die diese Zugänglichkeit nutzt die Signalisierungs-und Strukturierungs-Eigenschaften der ektodermalen Gewebe während Gesichts-Entwicklung zu testen. In diesen Experimenten, schaffen wir Wachtel-Küken 5 oder Maus-chick 6 Chimären durch Transplantation der Oberfläche cephalica Ektoderm, dass der Oberkiefer von Wachteln oder Maus deckt entweder auf der gleichen Region oder einer ektopischen Region Hühnerembryonen. Die Verwendung von Wachteln als Spendergewebe zur Transplantation in chicks wurde entwickelt, um die Vorteile eines nucleolar Marker in Wachteln, aber nicht chick Zellen tragen, sodass Ermittler zu hosten und zu Spendergewebe 7 unterscheiden. Ebenso ist eine sich wiederholende Element in der Maus-Genom und ist ubiquitär exprimiert, das uns erlaubt, Host-und Spendergewebe in Maus-chick Chimären 8 unterscheiden. Die Verwendung von Maus Ektoderm als Spendergewebe wird erheblich erweitern unser Verständnis dieser Gewebe-Wechselwirkungen, denn dies wird es uns ermöglichen, die Signalisierung Eigenschaften des Ektoderms aus verschiedenen mutierten Embryonen zu testen.

Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

Dieser Artikel beschreibt ein Gewebetransplantation Technik, die entwickelt, um die Signal-und Strukturierungs-Eigenschaften der Oberfläche cephalica Ektoderm während kraniofazialen Entwicklung Test war.

Die Beurteilung Signaling Eigenschaften von ektodermale Epithel Während Craniofacial Entwicklung

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