Name: assessing signaling properties of ectodermal epithelia during craniofacial development
Uploaded: 2011-03-24T11:00:00
Description: Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

この作品は、R01 - DE018234とR01 - DE019638によって賄われていた。

1。ドナー組織の準備<ol><li>培地を準備し、ガラスのピンをシャープに、タングステンの針を強めています。 </li><li>シェルから胚を回収、氷冷PBSで洗う。 <ol class="lalpha"><li> 10mLのシリンジと18ゲージの針を使用すると、卵の殻の先端からアルブミン1.0 mLを削除します。 </li><li>はさみのポイントを使用してシェルの上に小さな穴を確認し、胚を露出させる円形の開口部をカット</li></ol></li><li> DM...

<ol>
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この移植の方法を使用すると、私たちは外胚葉の背腹極性と上顎のproximodistal拡張を調節するシグナル伝達情報が含まれていることを決定することができました。ウズラやマウスの外胚葉、および多くの種6,11の間でこの組織の分子シグナルの保全を使用して結果の類似性は、これは脊椎動物間で高度に保存されたシグナル伝達の中心であることを示します。さらに、他の研究者らは、...

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assessing signaling properties of ectodermal epithelia during craniofacial development

鳥類胚のアクセシビリティは、実験的な発生学者が開発し、パターニングし、脊椎動物の形態形成（例えば、1、2、3、4）規制組織の相互作用の役割の間に細胞の運命を理解して支援してきました。ここで、我々は、顔の開発中に外胚葉組織のシグナリングとパターニング特性をテストするには、このアクセ​​シビリティを悪用する方法を示しています。これらの実験では、我々は、同じ地域やニワトリ胚の異所性の領域のいずれかの上にウズラやマウスからの上顎を覆う表面頭部外胚葉を移植することにより、ウズラ、ニワトリ5またはマウス-ひよこ6キメラを作成します。雛への移植のドナー組織としてのウズラの使用は、このように研究者がホストとドナーの組織7を区別できるように、ウズラの存在ではなく、ニワトリの細胞核マーカーを活用するために開発されました。私たちはマウスニワトリキメラ8のホストとドナーの組織を区別できるようになる、同様に、反復的な要素は、マウスのゲノムに存在し、遍在的に発現される。これは、私たちは様々な変異体胚に由来する外胚葉のシグナル伝達の特性をテストすることができますので、ドナーの組織としてのマウスの外胚葉の使用は大幅に、これらの組織の相互作用の理解を拡張します。

Watch this Scientific Journal Video about Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development at JoVE.com

Assessing Signaling Properties of Ectodermal Epithelia During Craniofacial Development

この記事では、頭蓋顔面の開発中にシグナリングと表面頭部外胚葉のパターニング特性をテストするために設計された組織移植の手法を説明します。

頭蓋顔面の開発時には外胚葉上皮のシグナリング特性を評価する

The accessibility of avian embryos has helped experimental embryologists understand the fates of cells during development and the role of tissue ...

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Human pluripotent stem cells (hPSCs) hold great promise for regenerative medicine and biopharmaceutical applications. Currently, optimal culture and ...

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The generation of chimeric embryos is a widespread and powerful approach to study cell fates, tissue interactions, and species-specific contributions to ...