Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/2574-v
March 28th, 2011
Chapters
0:05
Title
1:11
Principle of the Random Conical Tilt Method
2:10
Preparation of Holey Carbon Grids Covered with Thin Carbon
5:08
Negative Staining of the Exosome Complex
6:23
Electron Microscopy of the Exosome Complex
8:10
Image Processing of the Data
10:55
Representative Results for Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
11:29
Conclusion
この記事では、ネガティブ染色電子顕微鏡(EM)を用いて生体高分子の三次元(3D)再構成を得るために標準的な方法を説明します。このプロトコルでは、我々はランダムな円錐形の傾斜の再構成法(RCT)を使用して中解像度でサッカロマイセスセレビシエエキソソーム複合体の立体構造を取得する方法について説明します。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved