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DOI :
10.3791/2579-v
•
6:26 min
April 1st, 2011
Chapters
0:05
Title
1:19
Pericam-mt Transfection and Cell Preparation
4:11
Image Processing and Analysis
4:37
Fluorescence Imaging Results
5:58
Conclusion
本协议描述了线粒体钙通量实时荧光成像测量方法。该方法采用一个基于循环permutated YFP的双激励比例的钙传感器选择性表达在线粒体(比例pericam吨)的优势。
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