Name: spinal cord electrophysiology ii: extracellular suction electrode fabrication
Uploaded: 2011-02-20T23:59:00
Description: Watch this Scientific Journal Video about Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication at JoVE.com

塞缪尔百福是在索尔克生物研究所的基因表达实验室教授和霍华德休斯医学研究所研究员。这项工作是由克里斯托弗和Dana里夫基金会的支持。乔Belcovson，肯特Schnoeker和迈克沙利文在索尔克研究所的多媒体资源提供援助，摄影和剪辑。

隔离脊髓电生理记录可以揭示遗传和发育的变化的神经电路1。以前，我们表现出一个方法来解剖新生小鼠脊髓2。在这里，我们现在准备吸电极记录在孤立的 3个脊髓虚构运动有用的方法。 塑料管电极的尖端，可以得出一个手工非常精细的提示使用低温酒精灯3-5。塑料管材（PE90，粘土亚当斯Intramedic TM）举行了火焰软化成可塑性形式的?...

<ol>
	<li>Gallarda, B. W., Sharpee, T. O., Pfaff, S. L., Alaynick, W. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Defining+rhythmic+locomotor+burst+patterns+using+a+continuous+wavelet+transform.">Defining rhythmic locomotor burst patterns using a continuous wavelet transform.</a> Ann N Y Acad Sci. 1198, 133-139 (2010).</li><li>Meyer, A., Gallarda, B. W., Pfaff, S., Alaynick, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spinal+cord+electrophysiology.">Spinal cord electrophysiology.</a> J Vis Exp. , (2010).</li><li>Gallarda, B. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Segregation+of+axial+motor+and+sensory+pathways+via+heterotypic+trans-axonal+signaling.">Segregation of axial motor and sensory pathways via heterotypic trans-axonal signaling.</a> Science. 320, 233-236 (2008).</li><li>Landmesser, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+development+of+motor+projection+patterns+in+the+chick+hind+limb.">The development of motor projection patterns in the chick hind limb.</a> J Physiol. 284, 391-414 (1978).</li><li>Myers, C. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cholinergic+input+is+required+during+embryonic+development+to+mediate+proper+assembly+of+spinal+locomotor+circuits.">Cholinergic input is required during embryonic development to mediate proper assembly of spinal locomotor circuits.</a> Neuron. 46, 37-49 (2005).</li><li>Chanin, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+determination+of+chloride+by+use+of+the+silver-silver+chloride+electrode.">The determination of chloride by use of the silver-silver chloride electrode.</a> Science. 119, 323-324 (1954).</li><li>Goulding, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circuits+controlling+vertebrate+locomotion:+moving+in+a+new+direction.">Circuits controlling vertebrate locomotion: moving in a new direction.</a> Nat Rev Neurosci. 10, 507-518 (2009).</li></ol>

可以研究使用孤立的啮齿类动物脊髓神经系统的发育。在神经递质的存在，虚构的运动可以产生脊髓在图案的电气活动 1,3形式。这些节奏的阵阵生产0.2〜0.5 Hz时，在左，右和屈，伸交替图案。在不同的发展阶段，这项活动的稳定性和模式有所不同1。基因突变，也可以扰乱了这一活动的 3,5,7的图案。这项活动的转基因和发育的研究提供了组织的中枢模式产生电路的见解和?...

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<td>PTFE Sub Lite Wall Tubing (Small tubing)</td>
<td>&nbsp;</td>
<td>Zeus</td>
<td>36AWG</td>
<td>0.005&#x201D;ID x 0.003&#x201D; Wall (Small Parts) Also available in 0.003&#x201D; to 0.006&#x201D;</td>
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<td>Large tubing (0.86mm (0.34&#x201D;))</td>
<td>&nbsp;</td>
<td>Clay Adams Brand Intramedic Becton Dickinson and Company</td>
<td>427420</td>
<td>0.86mm (0.34&#x201D;) O.D. 1.27mm (.050&#x201D;)</td>
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<td>Electrode Barrel</td>
<td>&nbsp;</td>
<td>A-M Systems</td>
<td>573000</td>
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<td>Adhesive</td>
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<td>JB Weld</td>
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<td>Adhesive: Silicone caulk</td>
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<td>Bleach</td>
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<td>Xylene</td>
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<td>Silver wire: 0.010&#x201D;</td>
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<td>A-M Systems</td>
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<td>Insect pins: Austerlitz 0.1mm</td>
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<td>Fine Science Tools</td>
<td>26002-10</td>
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<td>Magnetic Stand</td>
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<td>Narishige</td>
<td>GJ-8</td>
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<td>Micromanipulator</td>
<td>&nbsp;</td>
<td>Narishige</td>
<td>MN 151</td>
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<td>Miniboard (Headstage)</td>
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<td>Grass Industries</td>
<td>F-15EB/B1</td>
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<td>Polyview Adaptor Unit</td>
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<td>Grass Industries</td>
<td>PVA 8</td>
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<td>Bipolar Portable Physiodata Amplifier System</td>
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<td>Grass Industries</td>
<td>15LT</td>
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<td>ANALOG TO DIGITAL CARD</td>
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<td>National Instruments</td>
<td>6035E</td>
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<td>Air Table; Vibraplane</td>
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<td>Kinetic Systems</td>
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<td>&nbsp;</td>		</tbody>
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spinal cord electrophysiology ii: extracellular suction electrode fabrication

使用新生儿啮齿类动物脊髓中枢模式发生器（CPG）的行为，可以研究神经设计电路和运动的发展。我们证明的方法来制造吸电极，用于检查中央人民政府的活动，或虚构的运动，在解剖啮齿类动物脊髓。被放置在人工脑脊液中的啮齿类动物脊髓和腹侧根绘制成吸电极。通过修改一个市售的吸电极的电极构造。较重的银线，是用来代替市售电极的标准线。为提高耐久性的塑料提示取代商业电极的玻璃尖。我们准备了手工绘制的电极和电极特定大小的管，允许的一致性和可重复性。数据收集，使用的放大器和neurogram数据采集软件。录音内执行一个法拉第笼，以防止机械和电气干扰，分别对空气表。

Watch this Scientific Journal Video about Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication at JoVE.com

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

外吸用来衡量新生儿啮齿类动物脊髓的电生理记录电极的制造和使用示范<em&gt;在体外</em

脊髓电二：外吸电极的制作

Development of neural circuitries and locomotion can be studied using neonatal rodent spinal cord central pattern generator (CPG) behavior. We demonstrate ...

Research

JoVE Journal

Neuroscience

Chicken Embryo Spinal Cord Slice Culture Protocol

鸡胚胎脊髓片培养协议

Slice cultures can facilitate the manipulation of  embryo development both pharmacologically and through gene manipulations. In  this reduced system, ...

科研

神经科学

Spinal Cord Transection in the Larval Zebrafish

在斑马鱼幼虫脊髓横断

Mammals fail in sensory and motor recovery following spinal cord injury due to lack of axonal regrowth below the level of injury as well as an inability ...

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time

一个<em&gt;离体</em&gt;激光诱导脊髓损伤模型，以评估轴索变性的机制在实时

Injured CNS axons fail to regenerate and often retract away from the injury site. Axons spared from the initial injury may later undergo secondary axonal ...

Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording

电从新生儿鼠害隔离脑干，脊髓准备允许呼吸中枢网络输出记录

While it is well known that the central respiratory drive is located in the brainstem, several aspects of its basic function, development, and response to ...

Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays

调查器官脊髓联合培养物中生长多电极阵列功能再生

Adult higher vertebrates have a limited potential to recover from spinal cord injury. Recently, evidence emerged that propriospinal connections are a ...

Spiral Ganglion Neuron Explant Culture and Electrophysiology on Multi Electrode Arrays

螺旋神经节神经元外植体培养及电多电极阵列

Spiral ganglion neurons (SGNs) participate in the physiological process of hearing by relaying signals from sensory hair cells to the cochlear nucleus in ...

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

脊髓神经元从新生小鼠中分离和培养

We present a protocol for the isolation and culture of spinal cord neurons. The neurons are obtained from neonatal C57BL/6 mice and are isolated on ...

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

胸腺1-gmcmp6 转基因小鼠在原代体血源皮质中的成像神经活性

The mammalian brain exhibits marked symmetry across the sagittal plane. However, detailed description of neural dynamics in symmetric brain regions in ...

Laminectomy and Spinal Cord Window Implantation in the Mouse

拉米内切手术和脊髓窗口植入小鼠

This protocol describes a method for spinal cord laminectomy and glass window implantation for in vivo imaging of the mouse spinal cord. An integrated ...

Activity-based Training on a Treadmill with Spinal Cord Injured Wistar Rats

脊髓损伤 wistar 大鼠跑步机的作业训练

Spinal cord injury (SCI) results in lasting deficits that include both mobility and a multitude of autonomic-related dysfunctions. Locomotor training (LT) ...