Name: patterning cells on optically transparent indium tin oxide electrodes
Uploaded: 2007-08-20T00:00:00
Description: Watch this Scientific Journal Video about Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes at JoVE.com

<p class="jove_title">第一部分：电极图案</p><ol><li>清洁和脱水的ITO镀膜玻璃载玻片涂有正性光刻胶</li><li>表面烤在100 ° C至除去溶剂从抗拒</li><li>烘烤后，表面暴露在紫外线光通过一个使用佳能光刻光罩</li><li>在显影液中暴露的地区被拆除</li><li>表面硬烤，以消除任何剩余的显影液</li><li>光阻图案的ITO保护区域被蚀刻掉在酸性腐蚀剂</li><li>剩余的光阻去除丙酮超声形成的ITO电极</li></ol><p class="jove_title">第二部分：表面?...

<ol>
	<li>Lee, J. Y., Jones, C., Zern, M. A., Revzin, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+Local+Tissue-Specific+Gene+Expression+in+Cellular+Micropatterns.">Analysis of Local Tissue-Specific Gene Expression in Cellular Micropatterns.</a> <em style='font-style: italic'> Analytical Chemistry</em>. <b>78</b>, 8305-8312 (2006).</li></ol>

<p>在这段视频中，我们已经演示了光学透明的铟锡氧化物电极的细胞图案。制造ITO电极采用光刻后，他们进行了修改，用聚乙二醇硅烷细胞电阻膜。单层脱附使用电化学开关电阻细胞表面细胞粘合。图案3T3小鼠成纤维细胞已被证明在他的视频。我们也有图案的肝细胞，原代细胞和星状细胞使用相同的技术。此外，由多个单独的电极，我们可以扩展这个技术组装多种细胞类型。此外，通过控制设计的几何形状，细胞?...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>Indium Tin Oxide</td>
<td>Other</td>
<td>Delta Technologies</td>
<td>CB 40IN</td>
<td>&nbsp;</td>
<tr>
<td>PEG silane</td>
<td>Reagent</td>
<td>Gelest Inc.</td>
<td>SIM6492.66</td>
<td>&nbsp;</td>
<tr>
<td>Hydrochloric Acid</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>320331</td>
<td>&nbsp;</td>
<tr>
<td>Nitric Acid</td>
<td>Reagent</td>
<td>Sigma Aldrich</td>
<td>258121</td>
<td>&nbsp;</td>
<tr>
<td>Ethanol</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>459836</td>
<td>&nbsp;</td>
<tr>
<td>Acetone</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>650501</td>
<td>&nbsp;</td>
<tr>
<td>Toluene</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>34866</td>
<td>&nbsp;</td>
<tr>
<td>Media</td>
<td>Reagent</td>
<td>Invitrogen Technologies</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Collagen</td>
<td>Reagent</td>
<td>Sigma-Aldrich</td>
<td>C3867</td>
<td>&nbsp;</td>
<tr>
<td>3T3 murine Fibroblasts</td>
<td>Cell Line</td>
<td>ATCC</td>
<td>&nbsp;</td>
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patterning cells on optically transparent indium tin oxide electrodes

<p class="jove_content">在本土组织的体外模拟的多细胞结构的组装能力行使的空间和时间的精确控制细胞表面的相互作用是一个重要前提。在这项研究中，光刻和湿法刻蚀技术用于制作在玻璃基板上可独立寻址的铟锡氧化物（ITO）电极。与聚乙二醇（PEG）的硅烷，使它们的蛋白质和细胞电阻的ITO微电极的玻璃基板进行了修改。聚乙二醇分子在电极表面的绝缘的存在是由用人钾铁氰化钾作为氧化还原记者分子的循环伏安核实。重要的是，还原电位的应用所造成的PEG层解吸，导致再生的导电电极表面出现典型的铁氰化钾氧化还原峰。还原潜在的应用也相当于从ITO电极属性交换细胞非细胞粘合胶粘剂。电化学剥离PEG硅烷层的ITO微电极细胞粘附允许地方在空间上定义的时尚，密切合作，以相应的电极模式的蜂窝模式。这些基板上展示了几种类型的细胞缩微。在将来，这种方法所提供的biointerfacial属性的控制将允许工程师通过三个或更多类型的细胞组装成精确的光学透明基板上的几何构型的细胞微环境。</p>

Watch this Scientific Journal Video about Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes at JoVE.com

Patterning Cells on Optically Transparent Indium Tin Oxide Electrodes

<p class="jove_content">不结垢PEG硅烷单层脱附从单独寻址的ITO玻璃电极由一个还原电位的应用。电化学剥离PEG硅烷层的ITO微电极细胞粘附允许地方在空间上定义的时尚，密切合作，以相应的电极模式的蜂窝模式。</p>

在光学透明的铟锡氧化物电极的图案细胞

The ability to exercise precise spatial and temporal control over cell-surface interactions is an important prerequisite to the assembly of multi-cellular ...

Research

JoVE Journal

Biology

Patterning of Embryonic Stem Cells Using the Bio Flip Chip

图案的使用生物倒装芯片的胚胎干细胞

Cell-cell interactions consisting of diffusible signaling and cell-cell contact (juxtacrine signaling) are important in numerous biological processes such ...

科研

生物学

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

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Glass microelectrodes (also called pipettes) have been a workhorse of electrophysiology for decades.  Today, such pipettes are made from glass capillaries ...

Fabrication of Amperometric Electrodes

安培电极的制备

Carbon fiber electrodes are crucial for the detection of catecholamine release from vesicles in single cells for amperometry measurements.  Here, we ...

Antifouling Self-assembled Monolayers on Microelectrodes for Patterning Biomolecules

防污图案生物分子自组装膜的微电极为

We present a procedure for forming a poly(ethylene glycol) (PEG) trimethoxysilane self-assembled monolayer (SAM) on a silicon substrate with gold ...

Analyzing the Function of Small GTPases by Microinjection of Plasmids into Polarized Epithelial Cells

为极化上皮细胞注射质粒的小GTP酶的功能分析

Epithelial cells polarize their plasma membrane into biochemically and functionally distinct apical and basolateral domains where the apical domain faces ...

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

从细胞中一氧化氮和超氧负离子自由基的电子顺磁共振光谱的检测，使用自旋陷阱

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Visualization of Mitochondrial Respiratory Function using Cytochrome <em>C</em> Oxidase / Succinate Dehydrogenase (COX/SDH) Double-labeling Histochemistry

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使用细胞色素线粒体呼吸功能的可视化<em> C</em>氧化酶/琥珀酸脱氢酶（COX / SDH），双标组织化学

Mitochondrial DNA (mtDNA) defects are an important cause of disease and may underlie aging and aging-related alterations 1,2. The mitochondrial theory of ...

Induction and Analysis of Epithelial to Mesenchymal Transition

诱导上皮和分析间质转化

Epithelial  to mesenchymal transition (EMT) is essential for proper morphogenesis during  development. Misregulation of this  process has been implicated ...

A Functional Assay for Gap Junctional Examination; Electroporation of Adherent Cells on Indium-Tin Oxide

对缝隙连接检查功能测定;贴壁细胞的氧化铟锡电

In this technique, cells are cultured on a glass slide that is partly coated with indium-tin oxide (ITO), a transparent, electrically conductive material. ...

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells

Application of Genetically Encoded Fluorescent Nitric Oxide NOÃƒÆ’Ã‚Â¢ÃƒÂ¢Ã¢â‚¬Å¡Ã‚Â¬Ãƒâ€šÃ‚Â¢ Probes, the geNOps, for Real-time Imaging of NOÃƒÆ’Ã‚Â¢ÃƒÂ¢Ã¢â‚¬Å¡Ã‚Â¬Ãƒâ€šÃ‚Â¢ Signals in Single Cells

遗传编码的荧光一氧化氮的应用（NO•）探针，所述geNOps，用于在单细胞NO•信号的实时影像

Nitric Oxide (NO&#8226;) is a small radical, which mediates multiple important cellular functions in mammals, bacteria and plants. Despite the existence ...