Name: rejection of fluorescence background in resonance and spontaneous raman microspectroscopy
Uploaded: 2011-05-18T12:00:00
Description: Watch this Scientific Journal Video about Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy at JoVE.com

Bu çalışma, NSF ödül DBI 0852891 tarafından finanse edildi. Bu çalışmanın bir parçası da Kooperatif Anlaşması No PHY0120999 altında, University of California, Davis tarafından yönetilen Biophotonics Bilim ve Teknoloji, NSF Bilim ve Teknoloji Merkezi Merkezi tarafından finanse edildi.

1. Bazı bakım, bu sistem içinde bir Raman numune hazırlama ve yerleştirme alınması gerekmektedir. <ol><li> Sistem genellikle çok kısa mesafelerde çok yüksek sayısal diyafram hedefleri kullanmak yapar, çünkü örnekler bir lamel üzerine yerleştirilir. Biyolojik numunelerin tipik bir Attofluor hücre odası (Invitrogen, Carlsbad, CA) monte No 1 kalınlığı lamel üzerine yerleştirilir. </li><li> Özellikle Sıvı numuneler, insanlar için toksik olan bir silikon epoksi sayesinde açılış çimentolu ...

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Biyomedikal Raman spektroskopisi alanında biyolojik teşhis çok zor zorlukları çözmek için gösterdi potansiyel bir sonucu olarak son birkaç yıl içinde artan bir ilgi gördü. Örneğin, Raman kanser tespiti 3, 4, 5, 6 tanısal değeri olduğu gösterilmiştir. Raman spektroskopisi bakteriyel kantitatif 7, 8 ve bakteriyel ilaç yanıtı 9 da kullanılır olmuştur. Ayrıca kemik sağlığı 10 Biyolojik Akışkanlar analizi 11, 12 arasında değişen diğer...

<table border="1"><tbody><tr><th> Isim </th><th> Şirket </th><th> Katalog Numarası </th><th> Yorumlar </th></tr><tr><td> Lensler </td><td> ThorLabs </td><td> Çeşitli </td><td> Her% 1 maksimum iletim kayıpları var kaplı tüm objektifler </td></tr><tr><td> Ayarlanabilir Ti: Sapph lazer </td><td> Tutarlı </td><td> Bukalemun </td><td> 30 NJ, 200, fs, 80 MHz </td></tr><tr><td> 40X yağ daldırma hedefi </td><td> Olympus </td><td> UApo/340 </td><td> NA = 1.35 </td></tr><tr><td> Ters mikroskop </td><td> Olympus </td><td> IX-71 </td><td> Yan port tüm objektifler kaldırmak için Modifiye </td></tr><tr><td> Yarım dalga plaka </td><td> Thorlabs </td><td> AHWP05M-600 </td><td></td></tr><tr><td> Glan-Tekin polarize </td><td> Thorlabs </td><td> GTH10M </td><td> ~% 10 iletim kaybı </td></tr><tr><td> Spektrometre </td><td> PI Acton </td><td> SP2300i </td><td></td></tr><tr><td> CCD </td><td> PI Acton </td><td> Pixis 100B </td><td></td></tr><tr><td> Mathmatical yazılım </td><td> MathWorks </td><td> MATLAB </td><td> versiyon 2008a </td></tr><tr><td> Faraday izolatör </td><td> EOT </td><td> BB8-5I </td><td></td></tr><tr><td> Piezo-elektrikli ayna </td><td> Newport </td><td> AG-M100 </td><td></td></tr><tr><td> BBO kristal </td><td> CASIX </td><td> özel </td><td> 1 mm kalınlık </td></tr><tr><td> Bandpass 1 </td><td> Andover </td><td> 008FC14 </td><td> 808 ± 0.4 nm </td></tr><tr><td> Dikroik ayna </td><td> Semrock </td><td> FF662-FDI01 </td><td> 662 nm bant kenarı </td></tr><tr><td> Uzun-pass filtre </td><td> Semrock </td><td> BLP01-405R </td><td> 417 nm bant kenarı </td></tr><tr><td> Bandpass filtresi 2 </td><td> Semrock </td><td> FF02-447/60 </td><td> 417-447 nm </td></tr><tr><td> CS 2 </td><td> Sigma-Aldrich </td><td> 335266 </td><td> % 99 saflıkta </td></tr><tr><td> Kumarin 30 </td><td> Sigma-Aldrich </td><td> 546127 </td><td> % 99 saflıkta </td></tr><tr><td> Immersiyon </td><td> Cargille </td><td> 16242 </td><td> Tip DF </td></tr></tbody></table>

rejection of fluorescence background in resonance and spontaneous raman microspectroscopy

Raman spektroskopisi, sık sık, özellikle biyolojik örnekler için, güçlü bir floresan arka plan boğulmuş. Hemen geç gelen floresan ışık Raman dağınık ışık gelen bir örnek, ultra hızlı bakliyat, zamansal zaman çizelgesi izole bir pikosaniye sinyalleri örtüşen Işıksal ayrı bir sistem bir tren ile heyecanlı. Burada Raman ve floresan sinyalleri izole etmek için, düşük güç optik Kerr kapı şeklinde tüm optik anahtarlama kullanan bir karmaşık nonlineer optik sistem inşası ve işletilmesi tartışacağız. &lt;5 mW Raman saçılması heyecanlandırmak için örnek gönderilen 404 nm ışık dönüştürülen 808 nm ışık yaklaşık 200 mW, ortalama güç ve 80 MHz tekrarlama oranı 2,4 W ile tek bir 808 nm lazer ikiye bölünmüş durumda. Kalan dönüştürülmemiş 808 nm ışık daha sonra, tüm optik deklanşör pompa gibi davranır doğrusal olmayan bir orta gönderdi. Deklanşör yaklaşık% 5 &#39;lik bir maksimum verimliliği ile 800 fs açar ve kapatır. Bu sistemi kullanarak, başarılı bir darbe enerjileri ve biyolojik olarak güvenli kalır ortalama güçleri kullanarak 80 MHz bir tekrarlama oranı Raman ve floresan sinyalleri birbirinden ayırmak mümkün. Sistem, optik güç açısından hiçbir yedek kapasite olduğundan, detaylı birçok tasarım ve hizalama konuları sisteminin verimini en üst düzeye yardım. Biz de içinde sinyal ve pompa kirişler Kerr orta mekansal ve zamansal örtüşme yanı sıra spektral edinimi için ayrıntılı bir protokol elde etmek için bizim protokol tartışmak. Sonuç olarak, zaman yolluk sistemi kullanarak güçlü floresan varlığında elde edilen Raman birkaç temsilcisi sonuçları raporu.

Watch this Scientific Journal Video about Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy at JoVE.com

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy

Biz ultrafast tüm optik anahtarlama floresan sinyallerine karşı Raman izole etmek için kullanan bir karmaşık doğrusal olmayan optik sistem inşası ve işletilmesi tartışacağız. Bu sistemi kullanarak, başarılı bir darbe enerjileri ve biyolojik olarak güvenli kalır ortalama yetkileri kullanan, Raman ve floresan sinyalleri ayrı edebiliyoruz.

Rezonans ve spontan Raman Microspectroscopy Floresan Arkaplan reddi

Raman spectroscopy is often plagued by a strong fluorescent background, particularly for biological samples. If a sample is excited with a train of ...

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