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DOI :
10.3791/2593-v
•
7:13 min
April 26th, 2011
Chapters
0:05
Title
1:26
Isolation of Nuclei
3:07
Isolation of Nucleosomes by in situ Micrococcal Nuclease Digestion
4:31
Nucleosome-ELISA (NU-ELISA)
6:45
Conclusion
核小体酶联免疫吸附(NU - ELISA法)是一个敏感的和定量的方法来检测翻译后修饰的全球格局,本土的,完整的核小体的筹备工作。这些修改包括甲基化,acetylations,以及在特定的组蛋白的氨基酸残基的磷酸化,因此NU - ELISA法提供了一个全球性的整体染色修改国家特定的细胞类型的蛋白质组检测。
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