Name: bioluminescence imaging for assessment of immune responses following implantation of engineered heart tissue (eht)
Uploaded: 2011-06-01T12:00:00
Description: Watch this Scientific Journal Video about Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT at JoVE.com

宋佳Schrepfer，莱纳德Conradi和Arne汉森收到由德意志研究联合会（DFG; SCHR992/3-1，SCHR992/4-1，二氧化碳858/1-1，医管局3423/3-1）的资金。这项工作是由来自欧盟托马斯Eschenhagen（EUGeneHeart和Angioscaff）的赠款支持。

1。东隧代和培养条件。 制造的纤维蛋白为基础的东隧，其他地方4所描述的。总之，生成纤维蛋白为基础的东隧植入的目的，主结构是包含从新生儿的ROSA /荧光素酶LEW转基因大鼠心脏，牛纤维蛋白原和基质胶细胞编制。琼脂糖压铸模具准备使用特氟隆垫片放置在6孔培养皿。琼脂糖凝固后，垫片被拆除，硅胶柱机架上培养皿放置到模具达到的职位。要生成东隧，mastermix?...

<ol>
	<li>Eschenhagen, T., Zimmermann, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+myocardial+tissue.">Engineering myocardial tissue.</a> Circ Res. 97, 1220-1231 (2005).</li><li>van der Bogt, K. E., Schrepfer, S., Yu, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+transplantation+of+adipose+tissue-+and+bone+marrow-derived+mesenchymal+stem+cells+in+the+infarcted+heart.">Comparison of transplantation of adipose tissue- and bone marrow-derived mesenchymal stem cells in the infarcted heart.</a> Transplantation. 87, 642-652 (2009).</li><li>Hakamata, Y., Murakami, T., Kobayashi, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term="Firefly+rats"+as+an+organ/cellular+source+for+long-term+in+vivo+bioluminescent+imaging.">"Firefly rats" as an organ/cellular source for long-term in vivo bioluminescent imaging.</a> Transplantation. 81, 1179-1184 (2006).</li><li>Hansen, A., Eder, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+Drug+Screening+Platform+Based+on+Engineered+Heart+Tissue.">Development of a Drug Screening Platform Based on Engineered Heart Tissue.</a> Circ Res. , (2010).</li></ol>

对心肌修复东隧技术的临床实施之前，根本问题，如细 ​​胞和基质成分或移植物存活的免疫原性植入后需要在活体生物发光成像实验模型进行评估。一个有用的新技术监测细胞的存活，移植后。我们成功地生成纤维蛋白为基础的东隧，含有荧光素酶（LUC）一个既定的协议4适应的心脏细胞。这些东隧植入受体鼠的大网膜。腹腔注射的特异底物（D -荧光素），产生的信号被检测到使...

<ul><li> LEW - TG（GT（ROSA）26Sor - LUC）11Jmsk大鼠（NBPR大鼠号码0299，http://www.anim.med.kyoto-u.ac.jp/NBR/） </li><li>牛纤维蛋白原（原液：200毫克/毫升加抑肽酶5微克/毫升纤维蛋白原在NaCl 0.9％，Sigma公司F4753） </li><li>基质胶（BD生物科学356235） </li><li> DMEM（F0415 Biochrome） </li><li>马血清（Gibco公司26050） </li><li>青霉素/链霉素（GIBCO 15140） </li><li>胰岛素（Σ- Aldrich公司I9278） </li><li>抑肽酶（西格玛爱秩序A1153） </li><li> D -荧光素（Biosynth L - 8220） </li><li>酶联免疫斑点（ELISPOT）检测板（Hassa Laborbedarf，S2EM004M99） </li></ul>

bioluminescence imaging for assessment of immune responses following implantation of engineered heart tissue (eht)

心脏组织工程的各种技术已经在过去几十年，包括使用本机或生物人工肝的支架材料，如纤维蛋白或胶原蛋白凝胶心肌细胞塌陷1心肌细胞单层堆放的脚手架战略追求。这些概念的目的是恢复心脏功能受损（如心肌梗死后），或作为实验模型（如预测毒理学和药物筛选或疾病建模）。精确监测细胞工程心脏组织（东隧）植入后的生存现在已经成为可能使用在体内的生物发光成像技术（BLI） 2。在这里，我们描述的纤维蛋白为基础的东隧从无处不在表达萤火虫荧光素酶（罗萨/荧光素酶LEW TG; 3）的转基因鼠应变的一代。植入的到不同的大鼠株的大网膜，以评估东隧植入受体生物体的免疫反应。 BLI和酶的酶联免疫斑点技术（ELISPOT）确认的评估免疫反应的可用性，BLI产生的结果比较。

Watch this Scientific Journal Video about Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT at JoVE.com

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

这个视频演示的使用<em&gt;在体内</em&gt;生物发光成像研究植入后大鼠的心脏组织工程（东隧）的免疫反应。

生物发光成像评估的免疫反应，植入心脏组织工程（东隧）

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

Research

JoVE Journal

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

磁共振弹性成像方法评价组织工程构造增长

Traditional mechanical testing often results in the destruction of the sample, and in the case of long term tissue engineered construct studies, the use ...

科研

生物工程

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves

协议相对液力评估的三单张聚合物阀

Limitations of currently available prosthetic valves, xenografts, and homografts have prompted a recent resurgence of developments in the area of ...

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

手术技术组织工程血管移植和后续的植入<em&gt;在体内</em&gt;监控

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

组织工程血管在小鼠模型的生成和嫁接

The construction of vascular conduits is a fundamental strategy for surgical repair of damaged and injured vessels resulting from cardiovascular diseases. ...

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model

三维组织工程人食管黏膜型号生产，表征和潜在用途

The incidence of both esophageal adenocarcinoma and its precursor, Barrett&#8217;s Metaplasia, are rising rapidly in the western world. Furthermore ...

Engineered 3D Silk-collagen-based Model of Polarized Neural Tissue

极化神经组织工程三维蚕丝胶原蛋白为基础的模式

Despite huge efforts to decipher the anatomy, composition and function of the brain, it remains the least understood organ of the human body. To gain a ...

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

A Combined 3D Tissue Engineered In Vitro/In Silico Lung Tumor Model for Predicting Drug Effectiveness in Specific Mutational Backgrounds

一种混合的3D组织工程<em&gt;体外</em&gt; /<em&gt;在硅片</em在具体的突变背景预测药物的有效性&gt;肺肿瘤模型

In the present study, we combined an in vitro 3D lung tumor model with an in silico model to optimize predictions of drug response based on a specific ...

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

人工程心脏组织的收缩自动分析心脏药物安全筛选

Cardiac tissue engineering describes techniques to constitute three dimensional force-generating engineered tissues. For the implementation of these ...

Custom Engineered Tissue Culture Molds from Laser-etched Masters

由激光蚀刻大师定制的组织培养模具

As the field of tissue engineering has continued to mature, there has been increased interest in a wide range of tissue parameters, including tissue ...

Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

小鼠模型中生物合成组织工程气管移植的育苗与植入

Treatment options for congenital or secondary long segment tracheal defects have historically been limited due to an inability to replace functional ...