Name: autologous blood injection to model spontaneous intracerebral hemorrhage in mice
Uploaded: 2011-08-24T12:00:00
Description: Watch this Scientific Journal Video about Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice at JoVE.com

这项工作是由一个转化医学和治疗学研究所的奖学金，并从医学和工程（T32HL007954）研究所的培训补助金，在美国宾夕法尼亚大学和马琳L.科恩和Jerome H. Fleisch学者格兰特在康涅狄格大学卫生中心（LHS）和国立卫生研究院NS - 029331（一汽）。

1。制备设备<ol><li>擦拭的立体框架，用75％乙醇和泵，以尽量减少细菌污染。 </li><li>消毒汉密尔顿注射器和熔融石英针。 注意：如果使用化学灭菌，使用前一定要在无菌水冲洗数次。 </li><li>石蜡纸的表面，并用75％乙醇擦拭，晾干。 </li></ol> 2。制备注射鼠标注意：有交付给你的动物设施在手术前至少7天，让他们适应环境，以新的环境和减轻压力的小?...

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	<li>Hua, Y., Schallert, T., Keep, R. F., Wu, J., Hoff, J. T., Xi, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+Tests+After+Intracerebral+Hemorrhage+in+the+Rat.">Behavioral Tests After Intracerebral Hemorrhage in the Rat.</a> Stroke. 33, 2478-2484 (2002).</li><li>James, M., Warner, D., Laskowitz, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preclinical+Models+of+Intracerebral+Hemorrhage:+A+Translational+Perspective.">Preclinical Models of Intracerebral Hemorrhage: A Translational Perspective.</a> Neurocritical Care. 9, 139-152 (2008).</li><li>Foerch, C., Arai, K., Jin, G., Park, K. -. P., Pallast, S., van Leyen, K., Lo, E. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+Model+of+Warfarin-Associated+Intracerebral+Hemorrhage.">Experimental Model of Warfarin-Associated Intracerebral Hemorrhage.</a> Stroke. 39, 3397-3404 (2008).</li><li>Nakamura, T., Xi, G., Hua, Y., Schallert, T., Hoff, J. T., Keep, R. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracerebral+hemorrhage+in+mice:+model+characterization+and+application+for+genetically+modified+mice.">Intracerebral hemorrhage in mice: model characterization and application for genetically modified mice.</a> Journal of Cerebral Blood Flow and Metabolism. 24, 487-494 (2004).</li><li>Rynkowski, M. A., Kim, G. H., Komotar, R. J., Otten, M. L., Ducruet, A. F., Zacharia, B. E., Kellner, C. P., Hahn, D. K., Merkow, M. B., Garrett, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mouse+model+of+intracerebral+hemorrhage+using+autologous+blood+infusion.">A mouse model of intracerebral hemorrhage using autologous blood infusion.</a> Nat. Protocols. 3, 122-128 (2008).</li><li>Wang, J., Fields, J., Dor&#233;, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+development+of+an+improved+preclinical+mouse+model+of+intracerebral+hemorrhage+using+double+infusion+of+autologous+whole+blood.">The development of an improved preclinical mouse model of intracerebral hemorrhage using double infusion of autologous whole blood.</a> Brain Research. 1222, 214-221 (2008).</li><li>Zhao, X., Sun, G., Zhang, J., Strong, R., Song, W., Gonzales, N., Grotta, J. C., Aronowski, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hematoma+resolution+as+a+target+for+intracerebral+hemorrhage+treatment:+Role+for+peroxisome+proliferator-activated+receptor+&#947;+in+microglia/macrophages.">Hematoma resolution as a target for intracerebral hemorrhage treatment: Role for peroxisome proliferator-activated receptor &#947; in microglia/macrophages.</a> Annals of Neurology. 61, 352-362 (2007).</li></ol>

这在小鼠的自发性基底节出血重现模型中使用自体尾动脉的血液结果脑出血的手术模型。在小鼠的脑出血模型提供的转基因动物研究病理生理学的可用性的优势，但其体积小，使神经外科手术技术上更比在较大的动物困难。 胶原酶模型和自体血液的注入模型实验性脑出血的两个行之有效的模型。虽然胶原酶模型提供了一个简单的过程和高度重现性出血 2，使用细菌蛋?...

鼠标神经外科立体定向框架（51925 Stoelting，） Microinfusion泵和处理器（UMP - 3和Micro4，世界精密仪器，萨拉索塔，佛罗里达州） 鼠标温暖（50300 Stoelting，） 鼠标吸入性麻醉（布伦特里科学，EZ - AF9000） 25μL，气密性高硼硅汉密尔顿涂层柱塞注射器和无针 （汉密尔顿公司，里诺，内华达州，1702RN注射器：765401，套圈：30949，垫片：30946） 熔融石英针切成2厘米长（汉密尔顿，17739） *注汉密尔顿注射器和熔融石英针可以重复使用多次手术，如果每次手术前消毒。这些材料是至关重要的，以避免血液凝固。 无菌手术手套手术衣，帽子和口罩优碘 75％的乙醇克针（一次性使用无菌27日） 无菌1毫升注射器（一次性使用） 无菌手术刀片 CIDEX 无菌水丁丙诺啡和异氟醚无菌纱布石蜡纸广场兽医外科胶（Vetbond，3M，明尼苏达州圣保罗）

autologous blood injection to model spontaneous intracerebral hemorrhage in mice

Investigation of the pathophysiology of injury after intracerebral
hemorrhage (ICH) requires a reproducible animal model. While ICH
accounts for 10-15% of all strokes, there remains no specific
effective therapy. The autologous blood injection model in mice
involves the stereotaxic injection of arterial blood into the basal
ganglia mimicking a spontaneous hypertensive hemorrhage in man. The
response to hemorrhage can then be studied in vivo and the
neurobehavioral deficits quantified, allowing for description of the
ensuing pathology and the testing of potential therapeutic agents. The
procedure described in this protocol uses the double injection
technique to minimize risk of blood reflux up the needle track, no
anticoagulants in the pumping system, and eliminates all dead space
and expandable tubing in the system.

Watch this Scientific Journal Video about Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice at JoVE.com

Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice

自体血注射在本议定书中所描述的小鼠脑出血模型，采用双色注塑技术，以最大限度地减少了针轨道，在泵送系统中没有抗凝血剂的血液回流的风险，并消除了所有死在空间和系统扩展的油管。

自体血液的注入模型小鼠自发性脑出血

Investigation of the pathophysiology of injury after intracerebral
hemorrhage (ICH) requires a reproducible animal model.  While ICH
accounts for 10-15% ...

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