Name: counting human neural stem cells
Uploaded: 2007-08-22T00:00:00
Description: Watch this Scientific Journal Video about Counting Human Neural Stem Cells at JoVE.com

<p>作者要感谢在儿童医院，奥兰治县提供hNSPC文化研究所的菲利普H ·施瓦茨博士国家人类神经干细胞资源。 </p>

<p class="jove_step">传代人类神经干细胞</p><p class="jove_content"> <em>注</em> ：请参阅的<strong class="jove_content"><a href="/index/details.stp?ID=263">传代人类神经干细胞</a></strong> <span class="jove_content">如何分离和悬浮细胞的文章</span> 。 <span class="jove_content">https://www.jove.com/index/Details.stp?ID=263</span> </p><p class="jove_step">准备用于计数细胞悬液</p><ol><li>将25μL0.4％台盼蓝溶液和20μL细胞介质到Eppendorf管。 </li><li>悬浮轻轻拍打管包含在媒体的?...

<ol>
	<li>Marchenko, S., Flanagan, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Passaging+Human+Neural+Stem+Cells.">Passaging Human Neural Stem Cells.</a> <em style='font-style: italic'>Journal of Visualized Experiments</em>. <b>7</b>, (2007).</li><li>Caprette, D. a. v. i. d. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+a+Hemacytometer.">Using a Hemacytometer.</a> <em style='font-style: italic'>BioEd Online</em>. , (2008).</li><li>Caprette, D. a. v. i. d. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Counting+Chamber+(Hemacytometer).">Counting Chamber (Hemacytometer).</a> <em style='font-style: italic'>BioEd Online</em>. , (2008).</li></ol>

<p>本协议描述了一个简单和快速的方式，各种涉及细胞的实验，以确定细胞浓度。使用在3D中描述的快捷方式，可以快速，准确地判断细胞浓度和细胞总数在给定的体积。 </p>

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counting human neural stem cells

<p class="jove_content">活细胞中的一个细胞悬液体积的确切数目的知识是必需的，如电镀细胞免疫细胞化学或细胞转染，许多日常的组织文化的操作。本协议描述了一个简单，快速的方法，区分活细胞和死细胞和量化的细胞浓度和细胞总数使用hemacytometer。这个过程首先需要分离从表面生长的细胞，他们在媒体resuspending。下一步，在台盼蓝（理想的浓度，将给予20-50％象限细胞）溶液稀释细胞，放在hemacytometer。最后，在随机选择的几个象限平均活菌计数，除以一个1 <sup>毫米</sup> 2象限（0.1μL），乘以稀释倍数的体积平均给每L.细胞的数量μL总体积乘以该细胞浓度，细胞总数。本协议描述了人类神经干/前体细胞（hNSPCs）计数，但也可以用于许多其他类型的细胞。 </p>

Watch this Scientific Journal Video about Counting Human Neural Stem Cells at JoVE.com

Counting Human Neural Stem Cells

<p class="jove_content">知识是需要许多组织培养操作活菌的确切人数。本协议描述了如何区分活细胞和死细胞和使用hemacytometer量化细胞。虽然它描述了人类神经干/前体细胞（hNSPCs）计数，它可用于其他类型的细胞。</p>

人类神经干细胞计数

Knowledge of the exact number of viable cells in a given volume of a cell suspension is required for many routine tissue culture manipulations, such as ...

Research

JoVE Journal

Biology

Propagation of Human Embryonic Stem (ES) Cells

Propagation of Human Embryonic Stem ES Cells

人类胚胎干细胞（ES细胞）的繁殖

科研

生物学

Transfecting Human Neural Stem Cells with the Amaxa Nucleofector

人类神经干细胞转染与Amaxa Nucleofector

Transfection of primary mammalian neural cells, such as human neural stem/precursor cells (hNSPCs), with commonly used cationic lipid transfection ...

Passaging Human Neural Stem Cells

传代人类神经干细胞

The ability to manipulate human neural stem/precursor cells (hNSPCs) in vitro provides a means to investigate their utility as cell transplants for ...

Immunocytochemistry: Human Neural Stem Cells

免疫组化：人类神经干细胞

Immunocytochemistry is a very powerful and fairly straightforward method for determining the presence, subcellular localization, and relative abundance of ...

Derivation of Human Embryonic Stem Cells by Immunosurgery

人类胚胎干细胞的推导Immunosurgery

The ability of human embryonic stem cells to self-renew and differentiate into all cell types of 
the body suggests that they hold great promise for both ...

Counting and Determining the Viability of Cultured Cells

计数和确定培养细胞的可行性

Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. A ...

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Mouse Epidermal Neural Crest Stem Cell EPI-NCSC Cultures

小鼠表皮神经嵴干细胞（EPI的非公务员合约雇员）文化

EPI-NCSC are remnants of the embryonic neural crest in an adult location, the bulge of hair follicles. They are multipotent stem cells that have the ...

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

从人类胚胎干细胞的染色质免疫沉淀

The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are ...

Culture and Maintenance of Human Embryonic Stem Cells  

文化和维护人类胚胎干细胞

Human embryonic stem (hES) cells must be monitored and cared for in order to maintain healthy, undifferentiated cultures. At minimum, the cultures must be ...

Freezing and Thawing Human Embryonic Stem Cells

人类胚胎干细胞冷冻和解冻

Since James Thomson et al developed a technique in 1998 to isolate and grow hES in culture, freezing cells for later use and thawing and expanding cells ...