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DOI :
10.3791/2631-v
•
12:52 min
March 3rd, 2011
Chapters
0:00
Title
2:56
Plating of Cells and Treatment with BMP
4:12
Fixation and Permeabilization
10:56
In Situ Proximity Ligation Assay Results from Neuro2a Cells Treated with BMP4
6:49
The Proximity Ligation Assay (PLA)
5:26
Blocking and Primary Antibodies Application
9:54
Preparation for Imaging
12:18
Conclusion
在这里,我们展示了如何使用接近结扎含量(解放军),与抗体结合的可视化骨形态发生蛋白(BMP),在固定的细胞信号。这种技术允许我们遵循激活效应的复合物的内源性BMP的核积累和BMP4的刺激下,他们的水平随着时间的推移进行量化。
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