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DOI :
10.3791/2631-v
•
12:52 min
March 3rd, 2011
Chapters
0:00
Title
2:56
Plating of Cells and Treatment with BMP
4:12
Fixation and Permeabilization
10:56
In Situ Proximity Ligation Assay Results from Neuro2a Cells Treated with BMP4
6:49
The Proximity Ligation Assay (PLA)
5:26
Blocking and Primary Antibodies Application
9:54
Preparation for Imaging
12:18
Conclusion
ここでは、骨形成タンパク質(BMP)固定された細胞のシグナル伝達を可視化する抗体の組み合わせで近接ライゲーションアッセイ(PLA)を、使用する方法を示します。このテクニックは、私たちは、内因性BMP活性化エフェクター複合体の核内蓄積を追跡し、BMP4刺激下で時間をかけて自分のレベルを定量することができました。
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