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DOI :
10.3791/2638-v
•
10:45 min
April 30th, 2011
Chapters
0:05
Title
1:19
UV Cross-linking of Tissue Culture Cells
2:10
Bead Preparation
2:53
Cell Lysis and Partial RNA Digestion
3:47
Immunoprecipitation
4:26
RNA 5' End Labelling
5:08
SDS-PAGE and Membrane Transfer
6:27
Gel Purification of cDNA
8:44
Representative Results
10:00
Conclusion
成绩单的RNA结合蛋白的空间安排,是一种转录后调控的关键因素。因此,我们制定个人核苷酸分辨率的紫外光交联和免疫(iCLIP),允许精确的全基因组RNA结合蛋白的结合位点的映射。
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