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DOI :
10.3791/2643-v
April 15th, 2011
Chapters
0:05
Title
1:06
Introduction
1:51
BiFC Calibration
3:04
Plating and Transfection of Cells
4:37
Imaging Cells
7:08
Monitoring SH3 Interactions of ITSN
8:13
Conclusion
在确定的时空调控细胞信号蛋白质的亚细胞定位是很重要的。在这里,我们描述了一个简单的方法监测在细胞内的蛋白质的空间相互作用的双分子荧光互补(附设)。
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