Name: engineering biological-based vascular grafts using a pulsatile bioreactor
Uploaded: 2011-06-14T12:00:00
Description: Watch this Scientific Journal Video about Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor at JoVE.com

यह काम स्वास्थ्य अनुदान EB-R01 ०,०८,८३६ के राष्ट्रीय संस्थानों और HL083895 R01 द्वारा वित्त पोषित है (दोनों और LEN के लिए). हम हमारे शोध के लिए बायोरिएक्टर बनाने के लिए, विश्वविद्यालय कंचेरा, डेरिल स्मिथ का शुक्रिया अदा करना सकता है.

आटोक्लेव इकट्ठा और प्रवाह प्रणाली और bioreactor घटकों (bioreactor खुद को और सिलिकॉन डाट ढक्कन) के रूप में चित्रा 1 और चित्रा 2 में निर्देश दिए के लिए टयूबिंग आटोक्लेव. दूध पिलाने की ट्यूब के एक छोर पर एक पुरुष...

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	<li>Risau, W., Flamme, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vasculogenesis.">Vasculogenesis.</a> Annu. Rev. Cell Dev. Biol. 11, 73-91 (1995).</li><li>Fankhauser, F., Bebie, H., Kwasniewska, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Influcence+of+mechanical+Forices+and+Flow+Mechanisms+on+Vessel+Occlusion.">The Influcence of mechanical Forices and Flow Mechanisms on Vessel Occlusion.</a> Lasers in Surgery and Medicine. 6, 530-532 (1987).</li><li>Niklason, L. E., Gao, J., Abbott, W. M., Hirschi, K. K., Houser, S., Marini, R., Langer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+arteries+grown+in+vitro.">Functional arteries grown in vitro.</a> Science. 284, 489-493 (1999).</li><li>Prabhakar, V., Grinstaff, M. W., Alarcon, J., Knors, C., Solan, A. K., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+porcine+arteries:+Effects+of+scaffold+modification.">Engineering porcine arteries: Effects of scaffold modification.</a> Journal of Biomedical Materials Research Part A. 67A, 303-311 (2003).</li><li>Mitchell, S. L., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Requirements+for+growing+tissue-engineered+vascular+grafts.">Requirements for growing tissue-engineered vascular grafts.</a> Cardiovascular Pathology. 12, 59-64 (2003).</li><li>Dahl, S. L. M., Rhim, C., Song, Y. C., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanical+properties+and+compositions+of+tissue+engineered+and+native+arteries.">Mechanical properties and compositions of tissue engineered and native arteries.</a> Annals of Biomedical Engineering. 35, 348-355 (2007).</li><li>Quint, C., Kondo, Y., Manson, R. J., Lawson, J. H., Dardik, A., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decellularized+tissue-engineered+blood+vessel+as+an+arterial+conduit.">Decellularized tissue-engineered blood vessel as an arterial conduit.</a> Proc Natl Acad Sci U S A. 108, 9214-9219 (2011).</li><li>Niklason, L. E., Yeh, A. T., Calle, E. A., Bai, Y., Valent&#237;n, A., Humphrey, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enabling+Tools+for+Engineering+Collagenous+Tissues+Integrating+Bioreactors,+Intravital+Imaging,+and+Biomechanical+Modeling.">Enabling Tools for Engineering Collagenous Tissues Integrating Bioreactors, Intravital Imaging, and Biomechanical Modeling.</a> Proceedings of the National Academy of Sciences of the United States of America. 107, 3335-3339 (2010).</li><li>Gong, Z., Calkins, G., Cheng, E. -. c., Krause, D., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+Culture+Medium+on+Smooth+Muscle+Cell+Differentiation+from+Human+Bone+Marrow-Derived+Mesenchymal+Stem+Cells.">Influence of Culture Medium on Smooth Muscle Cell Differentiation from Human Bone Marrow-Derived Mesenchymal Stem Cells.</a> Tissue Engineering Part A. 15, 319-330 (2009).</li><li>Gong, Z. D., Niklason, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small-diameter+human+vessel+wall+engineered+from+bone+marrow-derived+mesenchymal+stem+cells+(hMSCs.">Small-diameter human vessel wall engineered from bone marrow-derived mesenchymal stem cells (hMSCs.</a> Faseb Journal. 22, 1635-1648 (2008).</li><li>Poh, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+vessels+engineered+from+human+cells.">Blood vessels engineered from human cells.</a> Lancet. 365, 2122-2124 (2005).</li><li>American Heart Association. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Biostatistical fact sheet: cardiovascular procedures. , (2002).</li></ol>

इंजीनियर वाहिकाओं की गुणवत्ता के बड़े भाग में टिशू कल्चर में इस्तेमाल किया SMCs की गुणवत्ता से तय है. एसएमसी phenotype के महत्वपूर्ण पहलुओं सिकुड़ा आकारिकी, कम बीतने संख्या, और bioreactor अंदर पैदा करने की क्षमता शाम?...

<table border="1"><tbody><tr><th> अभिकर्मक / सामग्री के नाम </th><th> प्रदायक </th><th> सूचीपत्र संख्या </th></tr><tr><td> FBS (भ्रूण गोजातीय सीरम) हीट - निष्क्रिय </td><td> HyClone </td><td> SH30071 </td></tr><tr><td> DMEM </td><td> GIBCO </td><td> 11885 </td></tr><tr><td> rhFGF बुनियादी </td><td> आर एंड बी </td><td> 234-FSE </td></tr><tr><td> rrPDGF - बी.बी. </td><td> आर एंड बी </td><td> 520-बी.बी. </td></tr><tr><td> Penicilin जी </td><td> सिग्मा </td><td> PENNA </td></tr><tr><td> कॉपर सल्फेट (द्वितीय) </td><td> सिग्मा </td><td> C8027 </td></tr><tr><td> Gylcine </td><td> सिग्मा </td><td> C8790 </td></tr><tr><td> एल - Alanine </td><td> सिग्मा </td><td> A7469-25g </td></tr><tr><td> एल - Proline </td><td> सिग्मा </td><td> P5607-25g </td></tr><tr><td> Ascorbic एसिड </td><td> सिग्मा </td><td> A4544-25g </td></tr><tr><td> HEPES </td><td> सिग्मा </td><td> H3375-100g </td></tr><tr><td> सिलिकॉन डाट </td><td> कोल-Parmer </td><td> 06298-24 </td></tr><tr><td> Masterflex ट्यूबों एल / एस </td><td> कोल-Parmer </td><td> 06508-18 06508-16 </td></tr><tr><td> Masterflex पंप </td><td> कोल-Parmer </td><td> 7553-80 </td></tr><tr><td> Dacron कफ </td><td> Maquet </td><td> 174406 </td></tr><tr><td> पीजीए लगा </td><td> Concordia </td><td> MO000877-01 </td></tr><tr><td> 4-0 1.5 मीट्रिक Surgipro द्वितीय सीवन </td><td> Syneture </td><td> VP-557-X </td></tr><tr><td> 6-0 0.7 मीट्रिक Dexon सिवनी </td><td> Syneture </td><td> 7538-11 </td></tr><tr><td> 0.22μm PTFE फिल्टर </td><td> वाटमान </td><td> 6780-2502 </td></tr><tr><td> तीन रास्ता बंद करो - मुर्गा </td><td> एडवर्ड्स लाइफसाइंसेस </td><td> 593WSC </td></tr><tr><td> दबाव ट्रांन्सड्यूसर </td><td> एडवर्ड्स लाइफसाइंसेस </td><td> PX212 </td></tr><tr><td> चतुर्थ बैग </td><td> बैक्सटर </td><td> R4R2110 </td></tr><tr><td> खारा कमजोर पड़ने सेट </td><td> तीर </td><td> W20030 </td></tr><tr><td> सिलिकॉन टयूबिंग </td><td> Saint - Gobain </td><td> F05027 </td></tr></tbody></table>

engineering biological-based vascular grafts using a pulsatile bioreactor

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological environment, both mechanical and chemical stimulation are required to maintain the proper development and functionality of arterial vessels1,2.
Bioreactor culture systems developed by our group are designed to support vessel regeneration within a precisely controlled chemo-mechanical environment mimicking that of native vessels. Our bioreactor assembly and maintenance procedures are fairly simple and highly repeatable3,4. Smooth muscle cells (SMCs) are seeded onto a tubular polyglycolic acid (PGA) mesh that is threaded over compliant silicone tubing and cultured in the bioreactor with or without pulsatile stimulation for up to 12 weeks. There are four main attributes that distinguish our bioreactor from some predecessors. 1) Unlike other culture systems that simulate only the biochemical surrounding of native blood vessels, our bioreactor also creates a physiological pulsatile environment by applying cyclic radial strain to the vessels in culture. 2) Multiple engineered vessels can be cultured simultaneously under different mechanical conditions within a controlled chemical environment. 3) The bioreactor allows a mono layer of endothelial cells (EC) to be easily coated onto the luminal side of engineered vessels for animal implantation models. 4) Our bioreactor can also culture engineered vessels with different diameter size ranged from 1 mm to 3 mm, saving the effort to tailor each individual bioreactor to fit a specific diameter size. 
The engineered vessels cultured in our bioreactor resemble native blood vessels histologically to some degree. Cells in the vessel walls express mature SMC contractile markers such as smooth muscle myosin heavy chain (SMMHC)3. A substantial amount of collagen is deposited within the extracellular matrix, which is responsible for ultimate mechanical strength of the engineered vessels5. Biochemical analysis also indicates that collagen content of engineered vessels is comparable to that of native arteries6. Importantly, the pulsatile bioreactor has consistently regenerated vessels that exhibit mechanical properties that permit successful implantation experiments in animal models3,7. Additionally, this bioreactor can be further modified to allow real-time assessment and tracking of collagen remodeling over time, non-invasively, using a non-linear optical microscopy (NLOM)8. To conclude, this bioreactor should serve as an excellent platform to study the fundamental mechanisms that regulate the regeneration of functional small-diameter vascular grafts.

Watch this Scientific Journal Video about Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor at JoVE.com

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

हमारे समूह का एक bioreactor संस्कृति प्रणाली है कि हृदय प्रणाली के शारीरिक काँपने के गुणवाला तनाव implantable छोटे व्यास संवहनी grafts पुनर्जीवित करने के लिए mimics विकसित किया है.

इंजीनियरिंग जैविक आधार संवहनी Grafts का प्रयोग एक काँपने के गुणवाला bioreactor

Much effort has been devoted to develop and advance the methodology to regenerate functional small-diameter arterial bypasses. In the physiological ...

Research

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Bioengineering

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

ऊतक इंजीनियरिंग के लिए एक द्विअक्षीय यांत्रिक लोड हो रहा है बायोरिएक्टर का डिजाइन

We  designed a loading device that is capable of applying uniaxial or biaxial  mechanical strain to a tissue engineered biocomposites fabricated for  ...

Operation of a Benchtop Bioreactor

एक benchtop bioreactor के ऑपरेशन

Fermentation systems are used to provide an optimal growth environment for many different types of cell cultures. The ability afforded by fermentors to ...

Nonhuman Primate Lung Decellularization and Recellularization Using a Specialized Large-organ Bioreactor

There are an insufficient number of lungs available to meet current and future organ transplantation needs. Bioartificial tissue regeneration is an ...

Construction and Characterization of a Novel Vocal Fold Bioreactor

एक उपन्यास मुखर गुना bioreactor के निर्माण और विशेषता

In vitro engineering of mechanically active tissues requires the presentation of physiologically relevant mechanical conditions to cultured cells. To ...

Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System

एक एकल उपयोग वायवीय bioreactor प्रणाली का उपयोग स्तनधारी कोशिकाओं की खेती

Recent advances in mammalian, insect, and stem cell cultivation and scale-up have created tremendous opportunities for new therapeutics and personalized ...

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

ऊतक इंजीनियर संवहनी grafts और इसके बाद के आरोपण के लिए शल्य चिकित्सा तकनीक<em&gt; Vivo</em&gt; निगरानी

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

संवहनी ऊतक उत्थान के लिए इंजीनियरिंग 3 डी Cellularized कोलेजन जैल

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

विविध माइक्रोबियल समुदाय के उच्च घनत्व की खेती के लिए एक उपन्यास बायोरिएक्टर

A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Past ...

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

छवि निर्देशित, लेजर आधारित संवहनी व्युत्पन्न Microfluidic नेटवर्क का निर्माण

This detailed protocol outlines the implementation of image-guided, laser-based hydrogel degradation for the fabrication of vascular-derived microfluidic ...

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

इंजीनियर संवहनी grafts के स्केलिंग 3 डी मुद्रित गाइड और अंगूठी Stacking विधि का प्रयोग

Coronary artery disease remains a leading cause of death, affecting millions of Americans. With the lack of autologous vascular grafts available, ...