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DOI :
10.3791/2660-v
•
8:58 min
June 8th, 2011
Chapters
0:05
Title
1:27
Phosphorus Extraction and pH Adjustment
2:13
Enzyme Stock Preparation
2:59
P Calibration Curve and Controls
4:23
Sample + Enzyme Incubation
5:20
Colorimetric Measurement of Released and Background Inorganic P
6:09
Classification of P Compounds
7:35
HTP Analysis of Phosphorus in Soil
8:26
Conclusion
このプロトコルは、96サンプルのPモノエステル、Pのジエステルと無機P.アップが標準的な実験で一度に測定できるように土壌のリンを測定し、分類するためのマイクロプレートリーダーを利用して酵素加水分解のハイスループット法を説明します。
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