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DOI :
10.3791/2668-v
•
12:07 min
March 14th, 2011
Chapters
0:05
Title
1:14
Infection of CD4+ T-Cells with HIV-1
2:42
Purification of HIV-1 Infected Cells
3:41
CD107a Degranulation Assay
10:07
Evaluation of CD107a Degranulation and NK Cell Cytotoxicity
6:41
51Cr Release Assay
11:45
Conclusion
HIV感染細胞にナチュラルキラー細胞溶解反応を測定するために細胞毒性のアッセイは、標的細胞の純度によって制限されます。ここでは、CD4をダウンモジュレートするHIV - 1の能力を活用することにより、HIV - 1感染の主なT細胞芽球の高度に精製された人口の分離を示しています。
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