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DOI :
10.3791/2672-v
April 28th, 2011
Chapters
0:05
Title
1:05
Injection of Caged Fluorescein Dextran into Zebrafish Embryos
2:49
Laser Uncaging of Fluorescein Dextran
4:14
Antibody Labeling and Detection of Uncaged Fluorescein Dextran
6:36
Representative Immunolabeling Results
7:19
Conclusion
이 프로토콜은 uncaged 플루오레신에서 신호를 증폭하기 위해 immunolabeling 전체 산 뒤에 갇힌 플루오레신의 UV - uncaging를 사용하여 셀 zebrafish의 배아의 작은 그룹의 운명을 라벨 및 추적하는 방법을 delineates.
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