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DOI :
10.3791/2673-v
•
11:55 min
March 14th, 2011
Chapters
0:05
Title
2:00
Preparation of PBMC and Bulk Alloanergizing Cultures
3:57
Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction
6:48
Measuring the Specificity of Alloanergizing
9:05
Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation
9:43
Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR
10:34
Conclusion
本論文では、ヒト末梢血単核細胞にアロ抗原特異的アネルギーを誘発するための簡単なテクニックを説明します。技術は、非alloreactiveドナー細胞を生成するために臨床的に適用することができます。これらの細胞の注入は免疫再構築を改善し、同種造血幹細胞移植後の毒性を減らすことができる。
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