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JoVE Journal

Immunology and Infection

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Induction of Alloantigen-specific Anergy in Human Peripheral Blood Mononuclear Cells by Alloantigen Stimulation with Co-stimulatory Signal Blockade

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11:55 min

March 14th, 2011

March 14th, 2011

14,388 Views

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Title

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Preparation of PBMC and Bulk Alloanergizing Cultures

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Measuring the Efficacy of Co-Stimulatory Blockade and Alloanergizing by Mixed Lymphocyte Reaction

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Measuring the Specificity of Alloanergizing

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Measuring Proliferation in the Primary and Secondary MLRs by Thymidine Incorporation

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Results: Co-Stimulatory Blockade Reduces Proliferation in Primary MLR, and Alloanergizing PBMC Exhibit Decreased Proliferation in the Secondary MLR

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Conclusion

This paper describes a simple technique to induce alloantigen-specific anergy in human peripheral blood mononuclear cells. The technique can be applied clinically to generate non-alloreactive donor cells. Infusion of these cells could improve immune reconstitution and reduce toxicity after allogeneic hematopoietic stem cell transplantation.

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