Name: chromatin immunoprecipitation assay for tissue-specific genes using early-stage mouse embryos
Uploaded: 2011-04-29T16:00:00
Description: Watch this Scientific Journal Video about Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos at JoVE.com

这项工作是由美国国立卫生研究院R01 GM56244安仁，其中包括通过美国复苏与再投资法“，2009年授予的资金支持，由美国国立卫生研究院R01 GM87130 JARP

1。胚胎隔离 注 ：所有涉及小鼠的操作，应当按照相应的动物护理和使用的政策和协议执行<ol><li>检查雌性小鼠交配塞存在交配后的上午，他们放置在不同的笼子分开根根男性的交配女性。交配塞观察的一天中午，被认为是发展的0.5天（E0.5）胚胎。 </li><li>在E8.5，（或所需的阶段，如果有不同的），牺牲鼠标使用经批准的协议。 </li><li>湿安乐死动物用70％乙醇?...

<ol>
	<li>Minard, M. E., Jain, A. K., Barton, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+epigenetic+alterations+to+chromatin+during+development.">Analysis of epigenetic alterations to chromatin during development.</a> Genesis. 47, 559-572 (2009).</li><li>Kuo, M. H., Allis, C. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+cross-linking+and+immunoprecipitation+for+studying+dynamic+Protein:DNA+associations+in+a+chromatin+environment.">In vivo cross-linking and immunoprecipitation for studying dynamic Protein:DNA associations in a chromatin environment.</a> Methods. 19, 425-433 (1999).</li><li>Johnson, K. D., Bresnick, E. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dissecting+long-range+transcriptional+mechanisms+by+chromatin+immunoprecipitation.">Dissecting long-range transcriptional mechanisms by chromatin immunoprecipitation.</a> Methods. 26, 27-36 (2002).</li><li>Yusuf, F., Brand-Saberi, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+eventful+somite:+patterning,+fate+determination+and+cell+division+in+the+somite.">The eventful somite: patterning, fate determination and cell division in the somite.</a> Anat Embryol (Berl). 211, 21-30 (2006).</li><li>Buckingham, M., Bajard, L., Chang, T., Daubas, P., Hadchouel, J., Meilhac, S., Montarras, D., Rocancourt, D., Relaix, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+formation+of+skeletal+muscle:+from+somite+to+limb.">The formation of skeletal muscle: from somite to limb.</a> J Anat. 202, 59-68 (2003).</li><li>Wright, W. E., Sassoon, D. A., Lin, V. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myogenin,+a+factor+regulating+myogenesis,+has+a+domain+homologous+to+MyoD.">Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD.</a> Cell. 56, 607-617 (1989).</li><li>Edmondson, D. G., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+gene+with+homology+to+the+myc+similarity+region+of+MyoD1+is+expressed+during+myogenesis+and+is+sufficient+to+activate+the+muscle+differentiation+program.">A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.</a> Genes Dev. 3, 628-640 (1989).</li><li>Nabeshima, Y., Hanaoka, K., Hayasaka, M., Esumi, E., Li, S., Nonaka, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myogenin+gene+disruption+results+in+perinatal+lethality+because+of+severe+muscle+defect.">Myogenin gene disruption results in perinatal lethality because of severe muscle defect.</a> Nature. 364, 532-535 (1993).</li><li>Hasty, P., Bradley, A., Morris, J. H., Edmondson, D. G., Venuti, J. M., Olson, E. N., Klein, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Muscle+deficiency+and+neonatal+death+in+mice+with+a+targeted+mutation+in+the+myogenin+gene.">Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene.</a> Nature. 364, 501-506 (1993).</li><li>Sassoon, D., Lyons, G., Wright, W. E., Lin, V., Lassar, A., Weintraub, H., Buckingham, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+two+myogenic+regulatory+factors+myogenin+and+MyoD1+during+mouse+embryogenesis.">Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis.</a> Nature. 341, 303-307 (1989).</li><li>Brennan, T. J., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myogenin+resides+in+the+nucleus+and+acquires+high+affinity+for+a+conserved+enhancer+element+on+heterodimerization.">Myogenin resides in the nucleus and acquires high affinity for a conserved enhancer element on heterodimerization.</a> Genes Dev. 4, 582-595 (1990).</li><li>Rosenthal, N., Berglund, E. B., Wentworth, B. M., Donoghue, M., Winter, B., Bober, E., Braun, T., Arnold, H. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+highly+conserved+enhancer+downstream+of+the+human+MLC1/3+locus+is+a+target+for+multiple+myogenic+determination+factors.">A highly conserved enhancer downstream of the human MLC1/3 locus is a target for multiple myogenic determination factors.</a> Nucleic Acids Res. 18, 6239-6246 (1990).</li><li>Braun, T., Gearing, K., Wright, W. E., Arnold, H. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Baculovirus-expressed+myogenic+determination+factors+require+E12+complex+formation+for+binding+to+the+myosin-light-chain+enhancer.">Baculovirus-expressed myogenic determination factors require E12 complex formation for binding to the myosin-light-chain enhancer.</a> Eur J Biochem. 198, 187-193 (1991).</li><li>Chakraborty, T., Brennan, T., Olson, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+trans-activation+of+a+muscle-specific+enhancer+by+myogenic+helix-loop-helix+proteins+is+separable+from+DNA+binding.">Differential trans-activation of a muscle-specific enhancer by myogenic helix-loop-helix proteins is separable from DNA binding.</a> J Biol Chem. 266, 2878-2882 (1991).</li><li>French, B. A., Chow, K. L., Olson, E. N., Schwartz, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heterodimers+of+myogenic+helix-loop-helix+regulatory+factors+and+E12+bind+a+complex+element+governing+myogenic+induction+of+the+avian+cardiac+alpha-actin+promoter.">Heterodimers of myogenic helix-loop-helix regulatory factors and E12 bind a complex element governing myogenic induction of the avian cardiac alpha-actin promoter.</a> Mol Cell Biol. 11, 2439-2450 (1991).</li><li>Brennan, T. J., Chakraborty, T., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mutagenesis+of+the+myogenin+basic+region+identifies+an+ancient+protein+motif+critical+for+activation+of+myogenesis.">Mutagenesis of the myogenin basic region identifies an ancient protein motif critical for activation of myogenesis.</a> Proc Natl Acad Sci U S A. 88, 5675-5679 (1991).</li><li>Lassar, A. B., Davis, R. L., Wright, W. E., Kadesch, T., Murre, C., Voronova, A., Baltimore, D., Weintraub, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+activity+of+myogenic+HLH+proteins+requires+hetero-oligomerization+with+E12/E47-like+proteins+in+vivo.">Functional activity of myogenic HLH proteins requires hetero-oligomerization with E12/E47-like proteins in vivo.</a> Cell. 66, 305-315 (1991).</li><li>Chakraborty, T., Brennan, T. J., Li, L., Edmondson, D., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inefficient+homooligomerization+contributes+to+the+dependence+of+myogenin+on+E2A+products+for+efficient+DNA+binding.">Inefficient homooligomerization contributes to the dependence of myogenin on E2A products for efficient DNA binding.</a> Mol Cell Biol. 11, 3633-3641 (1991).</li><li>Cserjesi, P., Olson, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myogenin+induces+the+myocyte-specific+enhancer+binding+factor+MEF-2+independently+of+other+muscle-specific+gene+products.">Myogenin induces the myocyte-specific enhancer binding factor MEF-2 independently of other muscle-specific gene products.</a> Mol Cell Biol. 11, 4854-4862 (1991).</li><li>Braun, T., Arnold, H. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+four+human+muscle+regulatory+helix-loop-helix+proteins+Myf3-Myf6+exhibit+similar+hetero-dimerization+and+DNA+binding+properties.">The four human muscle regulatory helix-loop-helix proteins Myf3-Myf6 exhibit similar hetero-dimerization and DNA binding properties.</a> Nucleic Acids Res. 19, 5645-5651 (1991).</li><li>Serna, d. e. l. a., L, I., Ohkawa, Y., Berkes, C. A., Bergstrom, D. A., Dacwag, C. S., Tapscott, S. J., Imbalzano, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MyoD+targets+chromatin+remodeling+complexes+to+the+myogenin+locus+prior+to+forming+a+stable+DNA-bound+complex.">MyoD targets chromatin remodeling complexes to the myogenin locus prior to forming a stable DNA-bound complex.</a> Mol Cell Biol. 25, 3997-4009 (2005).</li><li>Blais, A., Tsikitis, M., Acosta-Alvear, D., Sharan, R., Kluger, Y., Dynlacht, B. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+initial+blueprint+for+myogenic+differentiation.">An initial blueprint for myogenic differentiation.</a> Genes Dev. 19, 553-569 (2005).</li><li>Cao, Y., Kumar, R. M., Penn, B. H., Berkes, C. A., Kooperberg, C., Boyer, L. A., Young, R. A., Tapscott, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+and+gene-specific+analyses+show+distinct+roles+for+Myod+and+Myog+at+a+common+set+of+promoters.">Global and gene-specific analyses show distinct roles for Myod and Myog at a common set of promoters.</a> EMBO J. 25, 502-511 (2006).</li><li>Ohkawa, Y., Yoshimura, S., Higashi, C., Marfella, C. G., Dacwag, C. S., Tachibana, T., Imbalzano, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myogenin+and+the+SWI/SNF+ATPase+Brg1+maintain+myogenic+gene+expression+at+different+stages+of+skeletal+myogenesis.">Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis.</a> J Biol Chem. 282, 6564-6570 (2007).</li><li>Davie, J. K., Cho, J. H., Meadows, E., Flynn, J. M., Knapp, J. R., Klein, W. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Target+gene+selectivity+of+the+myogenic+basic+helix-loop-helix+transcription+factor+myogenin+in+embryonic+muscle.">Target gene selectivity of the myogenic basic helix-loop-helix transcription factor myogenin in embryonic muscle.</a> Dev Biol. 311, 650-664 (2007).</li><li>Metivier, R., Penot, G., Hubner, M. R., Reid, G., Brand, H., Kos, M., Gannon, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen+receptor-alpha+directs+ordered,+cyclical,+and+combinatorial+recruitment+of+cofactors+on+a+natural+target+promoter.">Estrogen receptor-alpha directs ordered, cyclical, and combinatorial recruitment of cofactors on a natural target promoter.</a> Cell. 115, 751-763 (2003).</li><li>Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Struhl, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Current Protocols in Molecular Biology. , (2010).</li></ol>

在所描述的芯片协议，我们将展示生肌调节肌细胞生成素是在骨骼肌易制毒化学组织目前在单E8.5和E9.5胚胎肌细胞生成素推动者。此前的研究已经广泛的特点肌细胞生成素E盒含序列的结合，从最初在体外凝胶迁移实验利用体外翻译或细菌产生的肌细胞生成素和放射性标记的DNA编码的靶基因序列11-20监管的相关部分。传统的芯片研究已经证明了肌细胞生成素具有约束力的组织文...

<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Transitional//EN" "http://www.w3.org/TR/xhtml1/DTD/xhtml1-transitional.dtd">
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">	
		
		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>ChIP Assay Kit</td>
<td>&nbsp;</td>
<td>Upstate Cell Signaling Solutions, Millipore</td>
<td>17-295</td>
<td>&nbsp;</td>
<tr>
<td>Collagenase Type II</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>17101015</td>
<td>Dilution by 1 x PBS</td>
</tr>
<tr>
<td>Dulbecco&#x2019;s modified eagle medium (DMEM) </td>
<td>&nbsp;</td>
<td>Gibco Labs, Invitrogen</td>
<td>12100-061</td>
<td>High glucose content</td>
</tr>
<tr>
<td>Dulbecco&#x2019;s phosphate buffered saline 1X (DPBS)</td>
<td>&nbsp;</td>
<td>Gibco Labs, Invitrogen</td>
<td>14190-144</td>
<td>Calcium chloride free, Magnesium chloride free</td>
</tr>
<tr>
<td>Fetal bovine serum (FBS)</td>
<td>&nbsp;</td>
<td>Mediatech, Inc.</td>
<td>35-010-CV</td>
<td>&nbsp;</td>
<tr>
<td>Gel extraction kit</td>
<td>&nbsp;</td>
<td>QIAquick</td>
<td>28704</td>
<td>50 reaction kit</td>
</tr>
<tr>
<td>Penicillin/streptomycin stock solution</td>
<td>&nbsp;</td>
<td>Gibco Labs, Invitrogen</td>
<td>&nbsp;</td>
<td>5000 &mu;g/ml concentration</td>
</tr>
<tr>
<td>Protease Inhibitor Cocktail</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>P8340</td>
<td>&nbsp;</td>
<tr>
<td>Salmon sperm DNA /Protein A agarose</td>
<td>&nbsp;</td>
<td>Millipore</td>
<td>16-157</td>
<td>&nbsp;</td>
<tr>
<td>myogenin antibody</td>
<td>&nbsp;</td>
<td>Santa Cruz Biotechnology, Inc.</td>
<td>sc-576</td>
<td>&nbsp;</td>
<tr>
<td>Normal rabbit IgG</td>
<td>&nbsp;</td>
<td>Millipore</td>
<td>12-370</td>
<td>&nbsp;</td>
<tr>
<td>Platinum PCR Supermix</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>11306-016</td>
<td>&nbsp;</td>
<tr>
<td>GoTaq Q-PCR master mix</td>
<td>&nbsp;</td>
<td>Promega</td>
<td>A6001</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

chromatin immunoprecipitation assay for tissue-specific genes using early-stage mouse embryos

染色质免疫沉淀（ChIP）是一个强大的工具，以确定蛋白质：发生在活细胞的情况下1-3的染色质相互作用。这项技术已被广泛利用组织培养细胞中，并在较小程度上，在基层组织。啮齿类动物的胚胎组织，特别是在发展的早期，芯片的应用，是由组织的数量有限，异质性的胚胎细胞和组织类型复杂。在这里，我们提出了一种方法来执行芯片采用一个分离的胚胎每天8.5（E8.5）胚胎。剪切从单一E8.5胚胎的染色质可分为五个等份，它允许为控制和特定蛋白质的调查研究者足够的材料：染色质相互作用。 我们已经利用这种技术来开始：在组织特异性基因表达程序的规范文件蛋白质染色质相互作用。类型的细胞在胚胎的异质性必然限制了这项技术的应用，因为其结果是不区分的相互作用是否发生在所有检测的蛋白质：染色质相互作用的一个子集，或一个单一的细胞类型（S）。然而，考试期间或之后的组织特异性基因的组织特异性的基因表达发病原因有两个可行的。首先，一定免疫组织特定的因素隔离开来表达的细胞类型，其中的因素是染色质。其次，含有基因激活相关的翻译后修改的共激活因子和组蛋白的免疫应该只被发现在基因的细胞类型的基因和基因调控序列，或已被激活。这项技术应该适用于大多数组织特异性基因的激活事件的研究。 在这个例子中所述，我们利用E8.5和E9.5小鼠胚胎的研究在骨骼肌特定基因的启动子具有约束力的因素。体节，躯干和四肢骨骼肌肉就会形成易制毒化学组织，目前在E8.5 - 9.5 4,5。肌细胞生成素是一种调节因子骨骼肌分化6-9所需。数据表明，肌细胞生成素是与它自己的启动子在E8.5和E9.5胚胎。由于肌细胞生成素只在体节在这个发展的6,10阶段表示，数据表明，肌细胞生成素的相互作用与它自己的启动已经发生E8.5胚胎骨骼肌中的前体细胞。

Watch this Scientific Journal Video about Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos at JoVE.com

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

我们展示了染色质免疫沉淀（ChIP）的方法来识别组织特异性基因表达的组织特异性基因的小鼠胚胎组织中的发病因素相互作用期间或之后。此协议应广泛适用的组织特异性基因的激活的研究，因为它在正常胚胎发育过程中发生。

使用早期的小鼠胚胎组织特异性基因的染色质免疫沉淀检测

Chromatin immunoprecipitation (ChIP) is a powerful tool to identify protein:chromatin interactions that occur in the context of living cells 1-3. This ...

Research

JoVE Journal

Biology

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

配售早期鸡胚生长因子包被的磁珠

The neural tube expresses many proteins in specific spatiotemporal patterns during development.   These proteins have been shown to be critical for cell ...

科研

生物学

A Chromatin Assay for Human Brain Tissue

一个人脑组织中的染色质含量

Chronic neuropsychiatric illnesses such as schizophrenia, bipolar disease and autism are thought to result from a combination of genetic and environmental ...

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

从人类胚胎干细胞的染色质免疫沉淀

The functional and structural complexity of the myriad of cells in metazoan organisms arises from a small number of stem cells. Stem cells are ...

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

在激活人类细胞中的基因表达的动态检测组蛋白修饰染色质免疫沉淀（ChIP）

In response to a variety of extracellular ligands, the STAT (signal transducer and activator of transcription) transcription factors are rapidly recruited ...

Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay

在所有人类染色体24 chromosomics：检测的数值模拟和结构上的改动同时使用一种新型OctoChrome鱼含量

Fluorescence in situ hybridization (FISH) is a technique that allows specific DNA sequences to be detected on metaphase or interphase chromosomes in cell ...

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

Chromatin Immunoprecipitation ChIP using Drosophila tissue

染色质免疫沉淀（ChIP）使用果蝇组织

Epigenetics remains a rapidly developing field that studies how the chromatin state contributes to differential gene expression in distinct cell types at ...

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

生物质的使用限制金额高效的染色质免疫沉淀

Chromatin immunoprecipitation (ChIP) is a  widely-used method for determining the interactions of different proteins with  DNA in chromatin of living ...

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

代高通量测序技术（ChIP-seq的高品质的染色质免疫沉淀DNA模板）

ChIP-sequencing (ChIP-seq) methods directly  offer whole-genome coverage, where combining chromatin immunoprecipitation  (ChIP) and massively parallel ...

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

高效，快速的早期胚胎分离<em&gt;拟南芥</em&gt;种子

In flowering plants, the embryo develops within a nourishing tissue - the endosperm - surrounded by the maternal seed integuments (or seed coat). As a ...

Semi-automated Imaging of Tissue-specific Fluorescence in Zebrafish Embryos

组织特异性荧光的斑马鱼胚胎半自动成像

Zebrafish embryos are a powerful tool for large-scale screening of small molecules. Transgenic zebrafish that express fluorescent reporter proteins are ...