Name: an optic nerve crush injury murine model to study retinal ganglion cell survival
Uploaded: 2011-04-25T15:30:00
Description: Watch this Scientific Journal Video about An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival at JoVE.com

我们的研究是由美国国立卫生研究院，美国国家眼科研究所院内研究计划的支持。

所有的设备和试剂使用无菌。所有动物实验获得批准的动物护理和使用的聂/美国国立卫生研究院委员会（ACUC）（动物研究协议聂- 570），并根据美国国立卫生研究院的准则和法规执行。 1。基线逆行标记视网膜神经节细胞（RGC的）第1天这一程序的目的是标签的视网膜神经节细胞的神经示踪染料注射入小鼠视神经挤压伤的前三天，上丘逆行。染料将逆行采?...

<ol>
	<li>Yoles, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=GM1+reduces+injury-induced+metabolic+deficits+and+degeneration+in+the+rat+optic+nerve.">GM1 reduces injury-induced metabolic deficits and degeneration in the rat optic nerve.</a> Investigative ophthalmology &#38; visual science. 33, 3586-3591 (1992).</li><li>Fisher, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vaccination+for+neuroprotection+in+the+mouse+optic+nerve:+implications+for+optic+neuropathies.">Vaccination for neuroprotection in the mouse optic nerve: implications for optic neuropathies.</a> J Neurosci. 21, 136-142 (2001).</li><li>Levkovitch-Verbin, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=RGC+death+in+mice+after+optic+nerve+crush+injury:+oxidative+stress+and+neuroprotection.">RGC death in mice after optic nerve crush injury: oxidative stress and neuroprotection.</a> Investigative ophthalmology &#38; visual science. 41, 4169-4174 (2000).</li><li>Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VEGF-B+inhibits+apoptosis+via+VEGFR-1-mediated+suppression+of+the+expression+of+BH3-only+protein+genes+in+mice+and+rats.">VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats.</a> The Journal of clinical investigation. 118, 913-923 (2008).</li><li>Tang, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Survival+effect+of+PDGF-CC+rescues+neurons+from+apoptosis+in+both+brain+and+retina+by+regulating+GSK3beta+phosphorylation.">Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.</a> The Journal of experimental medicine. 207, 867-880 (2010).</li><li>Gustmann, S., Dunker, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo-like+organotypic+murine+retinal+wholemount+culture.">In vivo-like organotypic murine retinal wholemount culture.</a> J Vis Exp. , (2010).</li><li>Franklin, K., Paxinos, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Mouse Brain in Stereotaxic Coordinates. , (1997).</li></ol>

视神经挤压伤的小鼠模型研究RGC的死亡和生存的过程中是非常有用。这种模式也常常用于研究不同的试剂和RGC的凋亡和生存的基因的影响。这个模型的一个优点是它具有高度的重复性，最小的变化。 然而，需要特别照顾，在此模型中的几个步骤。首先，使视神经挤压伤时，它是必不可少的，不要用力过猛，而不是粉碎太久，因为它们可能导致眼动脉，因此随后的视网膜缺血?...

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<td>Stereotaxic apparatus</td>
<td>&nbsp;</td>
<td>ASI Instruments</td>
<td>SAS-4100</td>
<td>&nbsp;</td>
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<td>Dissecting microscope</td>
<td>&nbsp;</td>
<td>World Precision Instruments</td>
<td>PZMIV-BS </td>
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<td>Scalpel handle</td>
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<td>Fine Science Tools </td>
<td>10003-12 </td>
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<td>Scalpel Blades #15</td>
<td>&nbsp;</td>
<td>Fine Science Tools </td>
<td>10015-00</td>
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<td>Microdrill</td>
<td>&nbsp;</td>
<td>Fine Science Tools </td>
<td>18000-17</td>
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<td>Suture needle</td>
<td>&nbsp;</td>
<td>Fine Science Tools </td>
<td>12050-03</td>
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<td>Spring scissors</td>
<td>&nbsp;</td>
<td>Fine Science Tools </td>
<td>16144-13</td>
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<td>Dumont #5 forceps</td>
<td>&nbsp;</td>
<td>Fine Science Tools </td>
<td>11252-50</td>
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<td>Hamilton syringe</td>
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<td>Hamilton Company </td>
<td>88400</td>
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<td>Gauze sponges</td>
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<td>Office Depot</td>
<td>674889</td>
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<td>Fluorochrome</td>
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<td>Sigma</td>
<td>39286</td>
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<td>Sigma</td>
<td>459844</td>
<td>70%</td>
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<td>H2O2</td>
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<td>H3410</td>
<td>3%</td>
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an optic nerve crush injury murine model to study retinal ganglion cell survival

视神经损伤可导致轴索变性，逐渐死亡的视网膜神经节细胞（RGC的），在不可逆的视力丧失结果。在人类疾病的例子包括：外伤性视神经病变和青光眼视神经病变。它的特点是典型的视神经头的变化，逐步视神经病变，和视网膜神经节细胞的损失，如果不加控制，导致视力下降和失明。 视神经粉碎（ONC）损伤的小鼠模型，是一个重要的外伤性视神经病变的实验疾病模型，青光眼等，在此模型中，挤压伤到视神经，导致逐步视网膜神经节细胞的凋亡。这种疾病模型可以用来研究神经元死亡和生存，这是必要的治疗措施的发展的一般过程和机制。此外，药理学和分子的方法，可以在此模型中，识别和测试潜在的治疗试剂，治疗视神经病变的不同类型。 在这里，我们提供了一步一步演示（一）基线逆行标记视网膜神经节细胞（RGC的）第1天，（二）在4天的视神经挤压伤，（三）收获的视网膜和分析研资局在一天的生存11，（四）代表结果。

Watch this Scientific Journal Video about An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival at JoVE.com

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

该协议显示了如何逆行标记视网膜神经节细胞，以及如何随后视神经挤压伤，以分析视网膜神经节细胞的存活和凋亡。这是一个为不同类型的视神经病变，包括青光眼的实验性疾病模型。

一个视神经挤压伤的小鼠模型，研究视网膜神经节细胞生存

Injury to the optic nerve can lead to axonal degeneration, followed by a gradual death of retinal ganglion cells (RGCs), which results in irreversible ...

Research

JoVE Journal

Neuroscience

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

视神经横断：一个成人在中枢神经系统的神经元凋亡模型

Retinal ganglion cells (RGCs) are CNS neurons that output visual information from the retina to the brain, via the optic nerve. 
The optic nerve can be ...

科研

神经科学

An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells

一个孤立的视网膜制备，记录从基因标记的视网膜神经节细胞的光反应

The first steps in vertebrate vision take place when light stimulates the rod and cone photoreceptors of the retina 1.  This information is then ...

Minimally-invasive Technique for Injection into Rat Optic Nerve

微创技术注入到老鼠视神经

The rat optic nerve is a useful model for stem cell regeneration research. Direct injection into the rat optic nerve allows delivery into the central ...

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

在实验视神经脱髓鞘模型大鼠视觉诱发电位记录

The visual evoked potential (VEP) recording is widely used in clinical practice to assess the severity of optic neuritis in its acute phase, and to ...

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

视网膜神经节细胞定义子集的单细胞RNA-Seq

The discovery of cell type-specific markers can provide insight into cellular function and the origins of cellular heterogeneity. With a recent push for ...

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

背根神经节内注射和背根挤压伤为感官轴突再生模型

Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each ...

Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells

大鼠部分视神经切断术: 建立一种新的手术方法评价视网膜神经节细胞二次变性的模型

Previous studies have shown that the secondary degeneration of retinal ganglion cells (RGCs) occurs commonly in glaucoma. Partial optic nerve transection ...

Ethanol-Induced Cervical Sympathetic Ganglion Block Applications for Promoting Canine Inferior Alveolar Nerve Regeneration Using an Artificial Nerve

乙醇诱导的颈椎交交型神经节阻滞在促进犬下肺泡神经再生中的应用

Polyglycolic acid collagen (PGA-C) tubes are bio-absorbable nerve tubes filled with collagen of multi-chamber structure, which consist of thin collagen ...

Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration

大鼠模型中的面部神经外科研究同氧抑制与再生

This protocol describes consistent and reproducible methods to study axonal regeneration and inhibition in a rat facial nerve injury model. The facial ...

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

坐骨神经粉碎损伤大鼠模型中功能评估的3D运动学分析

Compared to the Sciatic Functional Index (SFI), kinematic analysis is a more reliable and sensitive method for performing functional evaluations of ...