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DOI :
10.3791/2686-v
June 6th, 2011
Chapters
0:05
Title
1:58
Egg Collection and Dechorionation
2:34
Microinjection with GDP-FucAI
3:30
BTTES-Cu(I)-catalyzed Click Chemistry Reaction
4:34
Imaging
5:06
In Vivo Imaging of Fucosylated Glycans During Zebrafish Embryogenesis
6:11
Conclusion
ゼブラフィッシュ胚におけるアルキンタグ付き糖鎖の迅速、非侵襲的、および堅牢なラベリングを可能にするクリックケミストリーベースの方法が記載されている。後期原腸形成の段階におけるゼブラフィッシュ胚の包絡層におけるフコシル化糖鎖は、本研究では画像化した。
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