Name: micro-dissection of rat brain for rna or protein extraction from specific brain region
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

<p class="jove_step">第1步</p><p class="jove_content">牺牲根据既定协议的大鼠。从头骨取出大脑和冲洗它在冰冷DEPC处理毫Q水，以消除任何表面的血液。 </p><p class="jove_step">第2步</p><p class="jove_content">放置在冰冷的金属板。剪下的大脑分为左，右半球双向一半。 <br /><br /><img src="/files/ftp_upload/old/269_2008_7_19_3/269_Figure-01.png" alt="269​​_Figure - 01" width="474" height="224" /></p><p style="PADDING-LEFT: 30px"> <strong>图1。从大鼠大脑半球的内侧，</s...

<ol>
	<li>Palkovits, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Maps and guide to micro-dissection of the rat brain</em>. , (c.1988).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Brain maps: structure of the rat brain. 3rd revised edition</em>. , (2004).</li></ol>

<p>不同的大脑区域不同的神经退行性疾病相关联。例如，额叶皮层和纹状体涉及与帕金森氏症与阿尔茨海默氏症有关，而海马。精确的大脑的微观解剖，使我们能够更准确地检测到病变部位的mRNA和蛋白的变化。 </p>

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micro-dissection of rat brain for rna or protein extraction from specific brain region

<p class="jove_content">到各地区的大鼠脑微解剖不同的神经退行性疾病的研究是极为重要的。本视频演示了微解剖四个主要的大脑区域包括嗅球，额叶皮质，纹状体和新鲜的大鼠脑组织海马。快速清除各自区域的有用的提示，以避免RNA和蛋白质组织的退化。</p>

Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

从特定的大脑区域的RNA或蛋白质提取大鼠脑微解剖

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

Research

JoVE Journal

Biology

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In this video, we demonstrate the procedure for isolating whole brains from adult Drosophila in preparation for recording from single neurons. We begin by ...

科研

生物学

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In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these ...

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In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously ...

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使用Bio - Rad公司的总RNA试剂盒从Neuroprecursor细胞的RNA提取

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制备大鼠脑组织神经元和神经胶质发展研究总结文化

An in vitro system that recapitulates the development and differentiation of progenitors into mature neurons and glia in the central nervous system (CNS) ...

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We proposed to investigate the gray matter reduction in the superior temporal gyrus seen in schizophrenia patients, by interrogating gene expression ...

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工程细胞透水蛋白

The protein transduction technique enables the direct delivery of biologically active material into mammalian cells [for review see 1,2]. For this one can ...

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毒素诱导和蛋白质提取<em>镰刀</em><em> SPP。</em蛋白质组学研究>文化

Fusaria are filamentous fungi able to produce different toxins. Fusarium mycotoxins such as deoxynivalenol, nivalenol, T2, zearelenone, fusaric acid, ...

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Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live ...

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从发展胚胎小鼠心脏瓣膜区蛋白分离

Western blot analysis is a commonly employed technique for detecting and quantifying protein levels. However, for small tissue samples, this analysis ...