Name: micro-dissection of rat brain for rna or protein extraction from specific brain region
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

<p class="jove_step"> Schritt 1 </p><p class="jove_content"> Sacrifice der Ratte nach festgelegten Protokollen. Entfernen Sie das Gehirn aus dem Schädel und spülen Sie ihn in eiskaltes DEPC behandelt Milli Q-Wasser auf jeder Oberfläche Blut zu entfernen. </p><p class="jove_step"> Schritt 2 </p><p class="jove_content"> Legen Sie an kalten Metallplatte. Cut das Gehirn bi-Hälfte in rechter und linker Gehirnhälfte. <br /><br /><img src="/files/ftp_upload/old/269_2008_7_19_3/269_Figure-01.png" alt="269_Figure-01" width="474" height="224" /></p>...

<ol>
	<li>Palkovits, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Maps and guide to micro-dissection of the rat brain</em>. , (c.1988).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Brain maps: structure of the rat brain. 3rd revised edition</em>. , (2004).</li></ol>

<p> Verschiedenen Hirnregionen assoziieren mit verschiedenen neurodegenerativen Erkrankungen. Zum Beispiel beziehen frontalen Kortex und Striatum mit Parkinson-Krankheit, während Hippocampus bezieht sich mit der Alzheimer-Krankheit. Präzise Mikro-Dissektion des Gehirns ermöglicht es uns, die mRNA-und Protein-Veränderungen in erkrankten Region genauer zu erfassen. </p>

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micro-dissection of rat brain for rna or protein extraction from specific brain region

<p class="jove_content">Micro-Dissektion Rattenhirn in verschiedenen Regionen ist äußerst wichtig für die Untersuchung von verschiedenen neurodegenerativen Erkrankungen. Dieses Video zeigt Mikrodissektion von vier großen Hirnregionen sind Riechkolben, frontalen Cortex, Striatum und Hippocampus in frischen Rattenhirngewebe. Nützliche Tipps für die schnelle Beseitigung von jeweiligen Regionen zu RNA-und Protein-Abbau des Gewebes zu vermeiden gegeben sind.</p>

Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

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