Name: micro-dissection of rat brain for rna or protein extraction from specific brain region
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

<p class="jove_step">ステップ1 </p><p class="jove_content">確立されたプロトコールに従って、ラットを生け贄に捧げる。頭蓋骨から脳を取り出して、表面の血液を除去するために、氷冷DEPC処理ミリQ水ですすいでください。 </p><p class="jove_step">ステップ2 </p><p class="jove_content">冷たい金属板の上に置きます。右と左半球に脳双方向の半分をカット。 <br /><br /><img src="/files/ftp_upload/old/269_2008_7_19_3/269_Figure-01.png" alt="269​​_Figure ...

<ol>
	<li>Palkovits, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Maps and guide to micro-dissection of the rat brain</em>. , (c.1988).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Brain maps: structure of the rat brain. 3rd revised edition</em>. , (2004).</li></ol>

<p>異なる脳の領域は異なる神経変性疾患に関連付ける。海馬はアルツハイマー病と関係しながら例えば、前頭皮質および線条体はパーキンソン病に関係する。脳の精密なマイクロダイセクションでは、私たちはより正確に病気の地域のmRNAと蛋白質の変化を検出することができます。 </p>

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micro-dissection of rat brain for rna or protein extraction from specific brain region

<p class="jove_content">様々な地域へのラット脳の微小解剖は、さまざまな神経変性疾患の研究のために極めて重要である。このビデオは4つの主要な脳領域の顕微解剖を示すことは新鮮なラット脳組織における嗅球、前頭皮質、線条体と海馬を含む。組織のRNAとタンパク質の分解を避けるために、それぞれの地域の迅速な除去のための有用なヒントが与えられている。</p>

Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

RNAまたは特定の脳領域からのタンパク質抽出用のラット脳の微小解剖

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

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Biology

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In chronic recording experiments, self-curing dental acrylic resins have been used as a mounting base of electrodes or microdialysis-probes. Since these ...

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In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously ...

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ニューロンとグリアの開発研究ラットの脳の集計文化の調製

An in vitro system that recapitulates the development and differentiation of progenitors into mature neurons and glia in the central nervous system (CNS) ...

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We proposed to investigate the gray matter reduction in the superior temporal gyrus seen in schizophrenia patients, by interrogating gene expression ...

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エンジニアリング細胞透過性のタンパク質

The protein transduction technique enables the direct delivery of biologically active material into mammalian cells [for review see 1,2]. For this one can ...

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から毒素誘導とタンパク質抽出<em>フザリウム属</em><em>属。</emプロテオーム研究>文化

Fusaria are filamentous fungi able to produce different toxins. Fusarium mycotoxins such as deoxynivalenol, nivalenol, T2, zearelenone, fusaric acid, ...

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Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live ...

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途上胚性マウス心臓弁地域からのタンパク質の単離

Western blot analysis is a commonly employed technique for detecting and quantifying protein levels. However, for small tissue samples, this analysis ...