Name: micro-dissection of rat brain for rna or protein extraction from specific brain region
Uploaded: 2007-08-30T00:00:00
Description: Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

<p class="jove_step"> Passo 1 </p><p class="jove_content"> Sacrificar o rato de acordo com protocolos estabelecidos. Remover o cérebro do crânio e lave-o em gelo frio DEPC tratados água Milli Q para remover qualquer vestígio de sangue de superfície. </p><p class="jove_step"> Passo 2 </p><p class="jove_content"> Coloque no prato de metal frio. Cortar o cérebro bi meio em hemisfério direito e esquerdo. <br /><br /><img src="/files/ftp_upload/old/269_2008_7_19_3/269_Figure-01.png" alt="01-269_Figure" width="474" height="224" /></p><p style=...

<ol>
	<li>Palkovits, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Maps and guide to micro-dissection of the rat brain</em>. , (c.1988).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Brain maps: structure of the rat brain. 3rd revised edition</em>. , (2004).</li></ol>

<p> Diferentes regiões do cérebro associada com diferentes doenças neurodegenerativas. Por exemplo, córtex frontal e striatum se relacionam com a doença de Parkinson, enquanto o hipocampo se relaciona com a doença de Alzheimer. Micro-dissecção precisa do cérebro que nos permite detectar as alterações mRNA e proteína na região de doentes com mais precisão. </p>

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micro-dissection of rat brain for rna or protein extraction from specific brain region

<p class="jove_content">Micro dissecção de cérebro de rato em várias regiões é extremamente importante para o estudo de diferentes doenças neurodegenerativas. Este vídeo demonstra dissecção de micro-regiões do cérebro de quatro principais incluem bulbo olfatório, córtex frontal, estriado e hipocampo em tecido fresco de cérebro de rato. Dicas úteis para remoção rápida de respectivas regiões para evitar a degradação de proteínas e RNA do tecido são dadas.</p>

Watch this Scientific Journal Video about Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region at JoVE.com

Micro-dissection of Rat Brain for RNA or Protein Extraction from Specific Brain Region

Micro-dissecção de cérebro de um rato para RNA ou Extração de Proteína da região específica do cérebro

Micro-dissection of rat brain into various regions is extremely important for the study of different neurodegenerative diseases. This video demonstrates ...

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