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DOI :
10.3791/2698-v
•
8:00 min
April 9th, 2011
Chapters
0:05
Title
1:23
Dissociation of Spinal Cord Tissue
2:47
Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
4:27
Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
6:16
Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
7:33
Conclusion
在流式细胞仪受伤/病理中枢神经系统的细胞炎症的定量分析是复杂的脂质/髓鞘碎片,可以有类似的大小和肉芽细胞,减少敏感性/准确性。我们拥有先进的一个细胞的制备方法去除髓鞘碎片和改善由流式细胞仪检测在脊髓损伤细胞检测。
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