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DOI :
10.3791/2698-v
•
8:00 min
April 9th, 2011
Chapters
0:05
Title
1:23
Dissociation of Spinal Cord Tissue
2:47
Creating OptiPrep Gradient Solutions and Separating Lipid/Myelin Debris from Cells
4:27
Immunolabeling of Specific Immune Cells and Flow Cytometric Quantitative Assessment
6:16
Removal of Lipid/Myelin Debris Enhances Quantitative Detection of Cellular Inflammation in the Injured Spinal Cord Tissue by Flow Cytometry
7:33
Conclusion
フローサイトメトリーによる負傷者/病理学的な中枢神経系における細胞性炎症の定量は感度/精度を低下させる、細胞に類似した大きさと造粒を持つことができる脂質/ミエリン破片によって複雑になる。我々は、ミエリン破片を除去し、損傷した脊髄のフローサイトメトリーによって細胞の検出を向上させるために細胞の調製法を進めてきた。
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