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DOI :
10.3791/2700-v
May 26th, 2011
Chapters
0:05
Title
1:00
Introduction
1:27
In vitro Fertilization of Xenopus Eggs
2:34
RNA Microinjection
3:46
Prepare Ectodermal Explants for Imaging
4:46
Alternative Approach: Prepare Cryosectioned Embryos for Imaging
5:57
Representative Fluorescent Imaging Results
6:29
Conclusion
非洲爪蟾胚胎外胚层已经成为一个有吸引力的模型研究细胞极性。描述一个试验,荧光蛋白的亚细胞分布在外胚层细胞进行评估。该协议将有助于地址问题涉及到空间的信号控制。
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