Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/2704-v
•
9:56 min
May 23rd, 2011
Chapters
0:05
Title
1:46
Introduction
2:23
Preparing the Stock Solutions for the Fluorescent Probes TMRM and H2DCF-DA
3:15
Loading Rat Cortical Neurons with TMRM and H2DCF-DA
4:42
Live Imaging of Neurons Incubated with TMRM to determine ΔΨm
6:17
Live Imaging of Neurons Incubated with H2DCF-DA to Determine ROS
7:18
Data Analysis
8:22
Representative Results: Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
9:33
Conclusion
我々は、特定の刺激の適用前と適用後のTMRM蛍光強度の相対的変化を決定するために皮質ニューロンで、蛍光指示薬、TMRMのアプリケーションを実証する。我々はまた、蛍光プローブHのアプリケーションを表示 2 DCF - DA皮質ニューロンにおける活性酸素種の相対的レベルを評価する。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved