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DOI :
10.3791/2707-v
•
9:18 min
May 19th, 2011
Chapters
0:05
Title
1:02
Setting Up the Laser Ablation System
2:17
Focusing the Laser: Z-plane
4:12
Focusing the Laser: X-Y Place
5:00
Immobilizing Worms for Laser Ablation
6:05
Performing Laser Axotomies
7:57
Scoring Neurons for Regeneration
8:32
Conclusion
에 신경을 절단하는 프로토콜 C. 엘레간스 MicroPoint 펄스 레이저가 함께 제공됩니다. 우리는 벌레를 immobilizing, 시스템을 설정하고, 레이블 뉴런을 severing 설명합니다. 장점은 상대적으로 낮은 비용으로 시스템 및 프로세스의 연결 또는 ablate 세포를 도려 수있는 능력을 포함 생체내에.
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