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DOI :
10.3791/2710-v
May 10th, 2011
Chapters
0:05
Title
0:45
In Situ Perfusion of Mouse Livers with Digestive Enzymes
2:37
In Vitro Digestion of Mouse Livers
3:10
Density Gradient Centrifugation
5:29
Results of Isolating Hepatic Stellate Cells
6:09
Conclusion
ここでは、マウスの肝臓から肝星細胞の単離のための方法を説明します。星状細胞の精製のために、マウスの肝臓は、消化されていますその場でと in vitroで密度勾配遠心分離の前にプロナーゼ、コラゲナーゼ処理による。この手法は、高純度の肝星細胞が得られます。
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