Name: magnetic resonance imaging quantification of pulmonary perfusion using calibrated arterial spin labeling
Uploaded: 2011-05-30T21:00:00
Description: Watch this Scientific Journal Video about Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling at JoVE.com

بدعم من المعاهد الوطنية للصحة HL081171 ، NIH HL080203

1. موضوع التعيين <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> موضوع السكان <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ويتم تجنيد الموضوعات عن طريق الإعلان لتناسب التركيبة السكانية المحددة المطلوبة للدراسة. </li><li style=";text-align:right;direction:rtl"> موضوع خاص لهذه الدراس...

<ol>
	<li>Bolar, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+regional+pulmonary+blood+flow+using+ASL-FAIRER.">Quantification of regional pulmonary blood flow using ASL-FAIRER.</a> Magn Reson Med. 55, 1308-1317 (2006).</li><li>Henderson, A. C., Prisk, G. K., Levin, D. L., Hopkins, S. R., Buxton, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterizing+pulmonary+blood+flow+distribution+measured+using+arterial+spin+labeling.">Characterizing pulmonary blood flow distribution measured using arterial spin labeling.</a> NMR Biomed. 22, 1025-1035 (2009).</li><li>Theilmann, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+MRI+measurement+of+lung+density+must+account+for+the+change+in+T(2)+(*)+with+lung+inflation.">Quantitative MRI measurement of lung density must account for the change in T(2) (*) with lung inflation.</a> J Magn Reson Imaging. 30, 527-534 (2009).</li><li>Hopkins, S. R., Garg, J., Bolar, D. S., Balouch, J., Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+blood+flow+heterogeneity+during+hypoxia+and+high-altitude+pulmonary+edema.">Pulmonary blood flow heterogeneity during hypoxia and high-altitude pulmonary edema.</a> Am J Respir Crit Care Med. 171, 83-87 (2005).</li><li>Miller, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Standardisation+of+spirometry.">Standardisation of spirometry.</a> Eur Respir J. 26, 319-338 (2005).</li><li>Hopkins, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vertical+gradients+in+regional+lung+density+and+perfusion+in+the+supine+human+lung:+the+Slinky+effect.">Vertical gradients in regional lung density and perfusion in the supine human lung: the Slinky effect.</a> J Appl Physiol. 103, 240-248 (2007).</li><li>Arai, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypoxic+pulmonary+vasoconstriction+does+not+contribute+to+pulmonary+blood+flow+heterogeneity+in+normoxia+in+normal+supine+humans.">Hypoxic pulmonary vasoconstriction does not contribute to pulmonary blood flow heterogeneity in normoxia in normal supine humans.</a> J Appl Physiol. 106, 1057-1064 (2009).</li><li>Dawson, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+pulmonary+vasomotion+in+physiology+of+the+lung.">Role of pulmonary vasomotion in physiology of the lung.</a> Physiol Rev. 64, 544-616 (1984).</li><li>Prisk, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+perfusion+in+the+prone+and+supine+postures+in+the+normal+human+lung.">Pulmonary perfusion in the prone and supine postures in the normal human lung.</a> J Appl Physiol. 103, 883-894 (2007).</li><li>Henderson, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steep+head-down+tilt+has+persisting+effects+on+the+distribution+of+pulmonary+blood+flow.">Steep head-down tilt has persisting effects on the distribution of pulmonary blood flow.</a> J Appl Physiol. 101, 583-589 (2006).</li><li>Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+age+on+pulmonary+perfusion+heterogeneity+measured+by+magnetic+resonance+imaging.">Effects of age on pulmonary perfusion heterogeneity measured by magnetic resonance imaging.</a> J Appl Physiol. 102, 2064-2070 (2007).</li><li>Wasserman, K. H., Sue, D., Casaburi, R., Whipp, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calculations,+Fomulae,+and+Examples+(Appendix+C).">Calculations, Fomulae, and Examples (Appendix C).</a> Principles of Exercise Testing and Interpretation. , (1999).</li></ol>

هذا الأسلوب يتيح قياس آثار تركيز الأوكسجين مستوحاة على التوزيع المكاني للتدفق الدم الرئوي باستخدام تقنيات الفسيولوجية الأساسية في البيئة تفحص MR. استخدام تقنيات الفيزيولوجية في تركيبة مع بروتون التصوير الكمية من الرئة نسبيا تنفيذها بسهولة. <p class="jove_content" style=";text-alig...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><th> معدات </th><th> شركة </th><th> نموذج </th></tr><tr><td> التصوير بالرنين المغناطيسي </td><td> جنرال الكتريك </td><td> 1.5 T GE HDX EXICITE twinspeed الماسح الضوئي </td></tr><tr><td> الأيض العربة </td><td> ParvoMedics </td><td> TrueOne 2400 </td></tr><tr><td> نبض oximeter </td><td> Nonin </td><td> 7500 FOR </td></tr><tr><td> مقياس التنفس </td><td> تقنيات طبية أندوفر </td><td> EasyOne diagonostic مقياس التنفس </td></tr><tr><td> قناع </td><td> هانز رودولف &amp; </td><td> 7400 سلسلة الأنف ووادي الذهب ، قناع ، الصغيرة والمتوسطة ، والكبيرة </td></tr><tr><td> صمام </td><td> هانز رودولف &amp; </td><td> اتجاهين غير إعادة التنفس الصمامات تي الشكل ™ التكوين ، 2600 متوسطة. كبير 2700 </td></tr><tr><td> تعيين رئيس </td><td> هانز رودولف &amp; </td><td> قلنسوة الرأس (حجم الكبار) ، وحزم والكليبات تأمين. </td></tr><tr><td> هوائي صمام التحكم في الاتجاهات وتحكم </td><td> هانز رودولف &amp; </td><td> واحدة من نوع المكبس انزلاق ™ وصمام تحكم 4285A </td></tr><tr><td> غير نشرها كيس لجمع الغاز </td><td> هانز رودولف &amp; </td><td> 6100 (100 لترا). </td></tr><tr><td> أنبوب </td><td> VacuMed </td><td> تنظيف أنابيب بور 108 &quot;، 1-3/8&quot; OD التجهيزات </td></tr><tr><td> الأشباح </td><td> معلمه </td><td> بريست زرع مستديرة ، 250CC </td></tr><tr><td> MATLAB </td><td> وMathWorks </td><td></td></tr></tbody></table>

magnetic resonance imaging quantification of pulmonary perfusion using calibrated arterial spin labeling

This demonstrates a MR imaging method to measure the spatial distribution of pulmonary blood flow in healthy subjects 
during normoxia (inspired O2, fraction (FIO2) = 0.21) hypoxia (FIO2 = 0.125), and hyperoxia 
(FIO2 = 1.00). In addition, the physiological responses of the subject are monitored in the MR scan environment. MR images 
were obtained on a 1.5 T GE MRI scanner during a breath hold from a sagittal slice in the right lung at functional residual capacity. An arterial 
spin labeling sequence (ASL-FAIRER) was used to measure the spatial distribution of pulmonary blood flow 1,2 and a multi-echo fast 
gradient echo (mGRE) sequence 3 was used to quantify the regional proton (i.e. H2O) density, allowing the quantification 
of density-normalized perfusion for each voxel (milliliters blood per minute per gram lung tissue). 
With a pneumatic switching valve and facemask equipped with a 2-way non-rebreathing valve, different oxygen concentrations 
were introduced to the subject in the MR scanner through the inspired gas tubing. A metabolic cart collected expiratory gas via expiratory tubing. Mixed expiratory O2 and CO2 concentrations, oxygen consumption, carbon dioxide production, respiratory exchange ratio, 
respiratory frequency and tidal volume were measured. Heart rate and oxygen saturation were monitored using pulse-oximetry. 
Data obtained from a normal subject showed that, as expected, heart rate was higher in hypoxia (60 bpm) than during normoxia (51) or hyperoxia (50) and the arterial oxygen saturation (SpO2) was reduced during hypoxia to 86%. Mean ventilation was 8.31 L/min BTPS during hypoxia, 7.04 L/min during normoxia, and 6.64 L/min during hyperoxia. Tidal volume was 0.76 L during hypoxia, 0.69 L during normoxia, and 0.67 L during hyperoxia. 
Representative quantified ASL data showed that the mean density normalized perfusion was 8.86 ml/min/g during hypoxia, 8.26 ml/min/g during normoxia and 8.46 ml/min/g during hyperoxia, respectively. In this subject, the relative dispersion4, an index of global heterogeneity, was increased in hypoxia (1.07 during hypoxia, 0.85 during normoxia, and 0.87 during hyperoxia) while the fractal dimension (Ds), another index of heterogeneity reflecting vascular branching structure, was unchanged (1.24 during hypoxia, 1.26 during normoxia, and 1.26 during hyperoxia). 
Overview. This protocol will demonstrate the acquisition of data to measure the distribution of pulmonary perfusion noninvasively under conditions of normoxia, hypoxia, and hyperoxia using a magnetic resonance imaging technique known as arterial spin labeling (ASL). 
Rationale: Measurement of pulmonary blood flow and lung proton density using MR technique offers high spatial resolution images which can be quantified and the ability to perform repeated measurements under several different physiological conditions. In human studies, PET, SPECT, and CT are commonly used as the alternative techniques. However, these techniques involve exposure to ionizing radiation, and thus are not suitable for repeated measurements in human subjects.

Watch this Scientific Journal Video about Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling at JoVE.com

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

يوصف نقص الأكسجين ، normoxia ، وفرط التأكسج ، : أسلوب التصوير بالرنين المغناطيسي لدراسة توزيع تدفق الدم الرئوي في إطار مجموعة من الظروف الفسيولوجية ، في هذه الحالة التعرض لثلاثة تركيزات مختلفة مستوحاة الأوكسجين. هذه التقنية تستخدم البحوث الفيزيولوجيا البشرية الرئوي التقنيات في بيئة المسح MR.

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This demonstrates a MR imaging method to measure the spatial distribution of pulmonary blood flow in healthy subjects 
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