Name: magnetic resonance imaging quantification of pulmonary perfusion using calibrated arterial spin labeling
Uploaded: 2011-05-30T21:00:00
Description: Watch this Scientific Journal Video about Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling at JoVE.com

支持HL081171美国国立卫生研究院，美国国立卫生研究院HL080203

1。除招聘<ol><li>除人口<ol><li>受试者招募广告，以满足研究所需的特定人群。 </li><li>这项研究的主题是健康，不吸烟 - 45岁至19岁之间的，没有心脏或肺部疾病的历史。 </li></ol></li><li>知情同意<ol><li>这项研究是由加州大学圣迭戈分校，人类研究保护项目批准。 </li><li>主题是通报了这项研究，如磁场曝光（M​​RI）和呼吸的缺氧和高氧的气体的潜在风险。 如果这个问题是女性生育能力的尿液...

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	<li>Bolar, D. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+regional+pulmonary+blood+flow+using+ASL-FAIRER.">Quantification of regional pulmonary blood flow using ASL-FAIRER.</a> Magn Reson Med. 55, 1308-1317 (2006).</li><li>Henderson, A. C., Prisk, G. K., Levin, D. L., Hopkins, S. R., Buxton, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterizing+pulmonary+blood+flow+distribution+measured+using+arterial+spin+labeling.">Characterizing pulmonary blood flow distribution measured using arterial spin labeling.</a> NMR Biomed. 22, 1025-1035 (2009).</li><li>Theilmann, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+MRI+measurement+of+lung+density+must+account+for+the+change+in+T(2)+(*)+with+lung+inflation.">Quantitative MRI measurement of lung density must account for the change in T(2) (*) with lung inflation.</a> J Magn Reson Imaging. 30, 527-534 (2009).</li><li>Hopkins, S. R., Garg, J., Bolar, D. S., Balouch, J., Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+blood+flow+heterogeneity+during+hypoxia+and+high-altitude+pulmonary+edema.">Pulmonary blood flow heterogeneity during hypoxia and high-altitude pulmonary edema.</a> Am J Respir Crit Care Med. 171, 83-87 (2005).</li><li>Miller, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Standardisation+of+spirometry.">Standardisation of spirometry.</a> Eur Respir J. 26, 319-338 (2005).</li><li>Hopkins, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vertical+gradients+in+regional+lung+density+and+perfusion+in+the+supine+human+lung:+the+Slinky+effect.">Vertical gradients in regional lung density and perfusion in the supine human lung: the Slinky effect.</a> J Appl Physiol. 103, 240-248 (2007).</li><li>Arai, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hypoxic+pulmonary+vasoconstriction+does+not+contribute+to+pulmonary+blood+flow+heterogeneity+in+normoxia+in+normal+supine+humans.">Hypoxic pulmonary vasoconstriction does not contribute to pulmonary blood flow heterogeneity in normoxia in normal supine humans.</a> J Appl Physiol. 106, 1057-1064 (2009).</li><li>Dawson, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+pulmonary+vasomotion+in+physiology+of+the+lung.">Role of pulmonary vasomotion in physiology of the lung.</a> Physiol Rev. 64, 544-616 (1984).</li><li>Prisk, G. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+perfusion+in+the+prone+and+supine+postures+in+the+normal+human+lung.">Pulmonary perfusion in the prone and supine postures in the normal human lung.</a> J Appl Physiol. 103, 883-894 (2007).</li><li>Henderson, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Steep+head-down+tilt+has+persisting+effects+on+the+distribution+of+pulmonary+blood+flow.">Steep head-down tilt has persisting effects on the distribution of pulmonary blood flow.</a> J Appl Physiol. 101, 583-589 (2006).</li><li>Levin, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+age+on+pulmonary+perfusion+heterogeneity+measured+by+magnetic+resonance+imaging.">Effects of age on pulmonary perfusion heterogeneity measured by magnetic resonance imaging.</a> J Appl Physiol. 102, 2064-2070 (2007).</li><li>Wasserman, K. H., Sue, D., Casaburi, R., Whipp, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Calculations,+Fomulae,+and+Examples+(Appendix+C).">Calculations, Fomulae, and Examples (Appendix C).</a> Principles of Exercise Testing and Interpretation. , (1999).</li></ol>

这种方法使测量吸入氧浓度的空间分布上的肺血流量，在MR扫描环境中使用基本的生理技术的影响。定量质子成像肺的生理技术结合使用是相对容易实现。 为了确保良好的质量测试，最重要的一步是培训的主体，屏气，在正确的肺容积和成像序列同步。由于ASL和质子密度图像上的FRC肺容积的可重复性依赖，任何膈肌或胸壁运动，会导致这些图像的位置不正。训练有素的科目是...

<table border="1"><tbody><tr><th>设备</th><th>公司</th><th>模型</th></tr><tr><td>磁共振成像</td><td>通用电气公司</td><td> 1.5 T GE HDX EXICITE twinspeed扫描器</td></tr><tr><td>代谢车</td><td> ParvoMedics </td><td> TrueOne 2400 </td></tr><tr><td>脉搏血氧仪</td><td>燕牌</td><td> 7500 </td></tr><tr><td>肺活量计</td><td>医疗技术安多弗</td><td> EasyOne diagonostic肺活量计</td></tr><tr><td>面膜</td><td>汉斯和鲁道夫</td><td> 7400系列口腔鼻面具，小型，中型，和大型</td></tr><tr><td>阀门</td><td>汉斯和鲁道夫</td><td>双向非再呼吸阀T形™配置，2600中等。 2700大</td></tr><tr><td>头紧定</td><td>汉斯和鲁道夫</td><td>头帽（成人的大小），带锁定夹。 </td></tr><tr><td>气动方向控制阀和控制器</td><td>汉斯和鲁道夫</td><td>单活塞滑动式™阀和控制器4285A </td></tr><tr><td>非扩散的气体收集袋</td><td>汉斯和鲁道夫</td><td> 6100（100升）。 </td></tr><tr><td>管</td><td> VacuMed </td><td>清洁博尔油管108“，1-3/8”外径配件</td></tr><tr><td>幻影</td><td>导师</td><td>布雷斯特植入回合，250cc组</td></tr><tr><td> MATLAB </td><td> The MathWorks公司</td><td></td></tr></tbody></table>

magnetic resonance imaging quantification of pulmonary perfusion using calibrated arterial spin labeling

这演示了磁共振成像的方法来衡量的肺血流量的空间分布在健康受试者在常氧（启发O 2的分数（F的第I O 2）= 0.21），缺氧（F的第I O 2 = 0.125），氧（F的第I O 2 = 1.00）。此外，主体的生理反应是在MR扫描环境监测。从矢状切片功能残气量在右肺呼吸保持在1.5 T GE核磁共振成像扫描仪在MR图像。动脉自旋标记序列（海拨更公平）是用来测量被用来量化区域的质子（即H 2 O的空间分布， 肺血流量1,2和多回波快速梯度回波序列（mGRE ）3）密度，使每个体素（毫升血液每分钟每克肺组织）的密度标准化灌注量化。 配备2路非再呼吸阀用气动开关阀和口罩，介绍了不同氧气浓度的磁共振扫描仪的主题，通过吸入气管。一个新陈代谢的车收集呼气时气体通过呼气管。混合呼气O 2和CO 2的浓度，氧气消耗，二氧化碳生产，呼吸交换率，呼吸频率和潮气量进行测量。使用脉冲血氧饱和度监测心率和血氧饱和度。从一个正常人获得的数据表明，正如所料，心率明显高于缺氧（60次），比在常氧（51）或氧（50）和动脉血氧饱和度（SPO 2）缺氧时减少到86％ 。平均通风是8.31升/分钟BTPS，在缺氧过程中，7.04升/分在常氧和6.64升/分钟时氧。潮气量为0.76大号在常氧，缺氧时，0.69大号过程中氧和0.67大号。 代表量化的ASL的数据显示，平均密度归灌注8.86毫升/分/克，8.26毫升/分/ G在常氧和8.46毫升/分/ G分别在高氧，缺氧时。在这个问题上，相对分散，全球异质性的指标 ，4增加了在缺氧（缺氧时为1.07，0.85在常氧，并在高氧0.87），而分形维数（DS），另一种异质性指数，反映血管的分支结构，不变（1.24缺氧时，在常氧1.26和1.26期间氧）。 概述。该协议将展示的数据采集来衡量使用磁共振成像技术被称为动脉自旋标记（ASL），常氧，缺氧，高氧条件下的非侵入性肺灌注的分布。 理由：肺血流量和肺质子密度，采用MR技术的测量提供高空间分辨率的图像，可以量化和执行几个不同的生理条件下重复测量的能力。在人类研究中，PET，SPECT和CT是常用的替代技术。然而，这些技术涉及暴露于电离辐射，因而不适合人类受试者重复测量。

Watch this Scientific Journal Video about Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling at JoVE.com

Magnetic Resonance Imaging Quantification of Pulmonary Perfusion using Calibrated Arterial Spin Labeling

一个磁共振成像方法研究各种生理条件下的肺血流量的分配，在这种情况下接触到三个不同吸入氧浓度，缺氧，常氧和氧，是描述。利用此技术在MR扫描环境中的人肺的生理学研究技术。

使用校准动脉自旋标记磁共振肺灌注显像定量

This demonstrates a MR imaging method to measure the spatial distribution of pulmonary blood flow in healthy subjects 
during normoxia (inspired O2, ...

Research

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Phase contrast magnetic resonance imaging (PC-MRI) is a noninvasive approach that can quantify flow-related parameters such as blood flow. Previous ...

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